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08:46 min
September 25th, 2010
DOI :
10.3791/2133-v
Chapters
0:00
Title
0:57
Introduction
1:21
Micropipette, DNA, and Embryo Preparation
4:15
Loading Micropipettes and Microinjection
6:49
Representative Injection Results
8:18
Conclusion
Summary
Using fine tip micropipettes we inject plasmid DNA into subdomains of chicken somites or neural tubes. The concentration of the plasmid is adjusted to generate single transfected cells. We then allow the cells to develop into clonal populations.
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