Name: an orthotopic model of serous ovarian cancer in immunocompetent mice for in vivo tumor imaging and monitoring of tumor immune responses
Uploaded: 2010-11-28T17:00:00
Description: Watch this Scientific Journal Video about An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses at JoVE.com

这项工作是支持由美国国立卫生研究院授予P01人工智能068730（SNC，NS），美国国立卫生研究院授予CA016520 / TAPITMAT（NS），从Claneil基金会（NS）的私人资金，和卵巢孢子授予“气候变化框架公约”和美国宾夕法尼亚大学（ P50 CA83638）和福克斯蔡斯癌症中心的核心格兰特（P30 CA06927）（DCC）的。作者感谢的光/生物发光博士EJ Delikatny执导，安东尼秘密的宾夕法尼亚大学在美国宾夕法尼亚州癌症大学博士G. Danet - Desnoyers定向干细胞和异种核心的核心，优秀的技术援助中心培训SNC的原位注射技术，在美国宾夕法尼亚州/ OCRC大学Denada Dangaj协助手术。

1。细胞培养<ol><li>前原位注射，文化MOV1 吉细胞，DR26肿瘤所得在T175烧瓶，直到他们90％融合。计划用1至5亿个细胞，每次注射，这将需要1或2 T175烧瓶。 </li><li>注射当天，收获细胞，并确定使用血球细胞数。 </li><li>一旦细胞浓度已经确定，颗粒细胞离心5分钟，在室温下在300 X克。 </li><li>旋转，悬浮细胞，在10微升无菌PBS 1万，0.002 M EDTA </li></ol> 2。手术前<ol><li>手术前?...

<ol>
	<li>Etzioni, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+case+for+early+detection.">The case for early detection.</a> Nat Rev Cancer. 3, 243-252 (2003).</li><li>Sasaroli, D., Coukos, G., Scholler, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beyond+CA125:+the+coming+of+age+of+ovarian+cancer+biomarkers.+Are+we+there+yet.">Beyond CA125: the coming of age of ovarian cancer biomarkers. Are we there yet.</a> Future Medicine. 3, 275-288 (2009).</li><li>Anderson, G. 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手术和原位注射卵巢囊原位注射要求的培训和精度。因此<ol><li>可怜的手术经验，实践与尸体第一。 </li><li>使用优先multiparous女性（一个或两个升）作为他们发展，随着时间的推移有利于注射和提高生存与未产妇女性更大卵巢。 </li><li>由于小鼠卵巢囊体积小，使用的最小可用针头大小的大力鼓励。 </li></ol> 在活体成像<ol><li>始终使用荧光，如1.5毫升...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>DMEM-GLUTAMAX</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>10564-011</td>
<td>&nbsp;</td>
<tr>
<td>PBS</td>
<td>&nbsp;</td>
<td>Gibco</td>
<td>14040</td>
<td>&nbsp;</td>
<tr>
<td>Versene</td>
<td>&nbsp;</td>
<td>Lonza</td>
<td>17-711E</td>
<td>&nbsp;</td>
<tr>
<td>Heating pad</td>
<td>&nbsp;</td>
<td>Deltaphase</td>
<td>39 DP</td>
<td>&nbsp;</td>
<tr>
<td>Povidone pads</td>
<td>&nbsp;</td>
<td>Dynarex</td>
<td>1108</td>
<td>&nbsp;</td>
<tr>
<td>Alcohol pads</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>06-669-62</td>
<td>&nbsp;</td>
<tr>
<td>Artificial tears ointment </td>
<td>&nbsp;</td>
<td>Phoenix Pharma., Inc.</td>
<td>17845-153</td>
<td>&nbsp;</td>
<tr>
<td>Ketoprofen</td>
<td>&nbsp;</td>
<td>Fort Dodge laboratories</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>3cc/insulin syringe</td>
<td>&nbsp;</td>
<td>BD</td>
<td>309301</td>
<td>&nbsp;</td>
<tr>
<td>Polyg Polyglycolic Acid suture/needle (3/8 19mm)</td>
<td>&nbsp;</td>
<td>Syneture</td>
<td>9612-31</td>
<td>&nbsp;</td>
<tr>
<td>Tissue adhesive</td>
<td>&nbsp;</td>
<td>Vetbond</td>
<td>3M</td>
<td>&nbsp;</td>
<tr>
<td>Vet Bactrim/ oral suspension</td>
<td>&nbsp;</td>
<td>Hi-tech Pharmacal</td>
<td>840823</td>
<td>&nbsp;</td>
<tr>
<td>IVIS-Lumina</td>
<td>&nbsp;</td>
<td>Caliper lifesciences</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Isofluorane</td>
<td>&nbsp;</td>
<td>Phoenix Pharma., Inc.</td>
<td>J108013</td>
<td>&nbsp;</td>
<tr>
<td>Fetal Bovine Serum, Qualified</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>10437036</td>
<td>&nbsp;</td>
<tr>
<td>Penicillin/streptomycin</td>
<td>&nbsp;</td>
<td>Gibco</td>
<td>15140</td>
<td>&nbsp;</td>
<tr>
<td>TurboFP635 mammalian vector</td>
<td>&nbsp;</td>
<td>Evrogen</td>
<td>FP721</td>
<td>&nbsp;</td>
<tr>
<td>T175 flasks</td>
<td>&nbsp;</td>
<td>cellstar</td>
<td>660-190</td>
<td>&nbsp;</td>		</tbody>
		</table>
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an orthotopic model of serous ovarian cancer in immunocompetent mice for in vivo tumor imaging and monitoring of tumor immune responses

背景：卵巢癌是一般诊断为晚期的情况下/病死率高，因此仍是最致命之间的美国妇女1,2,3的所有妇科恶性肿瘤。浆液性肿瘤卵巢癌的最普遍的形式和4,5 TG - MISIIR标签转基因是唯一的，自发地发展这种类型的肿瘤的小鼠模型。 TG - MISIIR标签小鼠表达SV40病毒转化地区的苗勒氏管抑制物质II型受体（MISIIR） 第 6基因启动子的控制之下。额外的转基因株系已经确定，表达转基因SV40的标签，但不发展卵巢肿瘤。非肿瘤易感小鼠C57BL / 6小鼠表现出典型的寿命和肥沃。这些小鼠可用于隔离TG - MISIIR - TAG - DR26小鼠肿瘤细胞同源移植。 目的：虽然肿瘤显像7，小动物活体深部肿瘤的早期发现是具有挑战性的。为了使浆液性卵巢癌的免疫完整的动物模型的临床前研究，我们描述了一个这种类型的卵巢癌，在活体成像，肿瘤微环境与肿瘤的免疫反应的研究许可证的同源小鼠模型。 方法：我们首先来自收获一种自发的在26周岁的DR26 TG - MISIIR标签的女性卵巢肿瘤标记+鼠标癌症细胞株（MOV1 ）。然后，我们稳定转导MOV1 TurboFP635的慢病毒哺乳动物载体，编码Katushka， 海葵Entacmaea quadricolor红色荧光蛋白635分之588 纳米 8,9,10极大激发/发射远红外突变细胞。我们原位植入MOV1 吉非肿瘤容易发生TG - MISIIR标签女性小鼠卵巢11,12,13,14。其次是在体内光学成像和肿瘤微环境是由免疫组织化学分析的肿瘤进展。 结果：原位植入MOV1 吉细胞浆液性卵巢肿瘤。 MOV1 吉肿瘤可以在活体成像的可视化由植入后3周（图1），并与白细胞浸润，15（图2）在人类卵巢癌的观察。 结论：我们描述了一个卵巢癌原位模型， 适合在高pH值的稳定，并在深部组织Katushka耐光由于早期肿瘤的体内成像。我们建议使用这种新颖的浆液性卵巢癌的同源模型，在活体成像的研究和监测肿瘤的免疫反应和免疫疗法。

Watch this Scientific Journal Video about An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses at JoVE.com

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

研究<em&gt;在体内</em&gt;肿瘤的生长和肿瘤微环境中，我们用在免疫动物的同源和原位卵巢癌的小鼠模型。我们转Katushka荧光蛋白（MOV1一只老鼠的肿瘤细胞株（MOV1）<sup&gt; KAT</sup&gt;），我们在这里展示其在卵巢原位移植和<em&gt;在体内</em&gt;成像。

浆液性卵巢癌原位模型在免疫小鼠，为在体内肿瘤显像和肿瘤免疫反应的监测

Background: Ovarian cancer is generally diagnosed at an advanced stage where the case/fatality ratio is high and thus remains the most lethal of all ...

Research

JoVE Journal

Immunology and Infection

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

Murine Model of CD40-activation of B cells

激活的B细胞CD40的小鼠模型

Research on B cells has shown that CD40 activation improves their antigen presentation capacity. When stimulated with interleukin-4 and CD40 ligand ...

科研

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一个<em&gt;体外</em&gt;模型在HIV合并感染的背景下测量免疫应答疟疾

Malaria and HIV co-infection is a growing health priority. However, most research on malaria or HIV currently focuses on each infection individually. ...

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IL-8瞬时转化的小鼠模型<em&gt;体内</em&gt;长期监测炎症反应

Airway inflammation is often associated with bacterial infections and represents a major determinant of lung disease. The in vivo determination of the ...

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

Salmonella typhimurium is a facultative intracellular bacterium that causes gastroenteritis in humans. After invasion of the lamina propria, S. ...

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

活体细胞毒性试验研究肿瘤特异性 CD8+ t 细胞反应中的免疫调节

Carboxyfluorescein succinimidyl ester (CFSE)-based in vivo cytotoxicity assays enable sensitive and accurate quantitation of CD8+ cytolytic T lymphocyte ...

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

小鼠中人类胸膜皮质瘤的PET/CT的植入与监测

Malignant pleural mesothelioma (MPM) is a rare and aggressive tumor arising in the mesothelium that covers the lungs, the heart, and the thoracic cavity. ...

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

肿瘤微环境下免疫细胞种群的可视化、定量和映射

The immune landscape of the tumor microenvironment (TME) is a determining factor in cancer progression and response to therapy. Specifically, the density ...

Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice

精确的大脑映射，在小鼠中执行神经-免疫动力学的重复体内振动成像

The central nervous system (CNS) is regulated by a complex interplay of neuronal, glial, stromal, and vascular cells that facilitate its proper function. ...

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

用于剖析体外肿瘤微环境中免疫反应的微流控共培养模型

Complex disease models demand cutting-edge tools able to deliver physiologically and pathologically relevant, actionable insights, and unveil otherwise ...

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

由加德纳菌阴道膀胱暴露在小鼠中引发的复发性大肠杆菌尿路感染

Recurrent urinary tract infections (rUTI) caused by uropathogenic Escherichia coli (UPEC) are common and costly. Previous articles describing models of ...