Name: injection of dsrna into female a. aegypti mosquitos
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Injection of dsRNA into Female A. aegypti Mosquitos at JoVE.com

<ol><li> Maak micro-injectie naalden door te trekken glazen capillaire buizen. We maken gebruik van een naald trekker van Sutter Instrument CO, (Model P-2000). De naald trekker programma specificaties zijn: warmte: 275 fil: 3 vel: 37 del: 250 pul: 125 </li><li> Plaats een bekend volume (bv. 1 pi) van water in de naalden en handmatig de schaal markeren met een fijne Sharpie. </li><li> Het opzetten van een post-injectie beker voor de muggen. Eerste snede het uiterste puntje af van een 1000 ui p...

injection of dsrna into female a. aegypti mosquitos

Omgekeerde genetische benaderingen hebben bewezen zeer nuttig voor het bepalen van welke genen de basis liggen van resistentie tegen pathogenen in vector muggen. Deze video protocol illustreert een methode die wordt gebruikt door de James lab dsRNA injecteren in vrouwelijke A. aegypti muggen die de dengue virus haven. De techniek voor het kalibreren van injectienaalden, het manipuleren van de injectie setup, en het injecteren van dsRNA in de thorax wordt geïllustreerd.

Watch this Scientific Journal Video about Injection of dsRNA into Female A. aegypti Mosquitos at JoVE.com

Injection of dsRNA into Female A. aegypti Mosquitos

Injectie van dsRNA in Vrouw A. aegypti Mosquitos

Reverse genetic approaches have proven extremely useful for determining  which genes underly resistance to vector pathogens in mosquitoes.  This video ...

Research

JoVE Journal

Biology

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

Micro-injectie van A. aegypti embryo&#39;s om transgene muggen verkrijgen

In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue ...

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

Protocol for Dengue Infections in Mosquitoes A. aegypti and Infection Phenotype Determination

Protocol voor Dengue Infecties in Muggen (A. aegypti) en Infectie Fenotype Bepaling

The purpose of this procedure is to infect the Aedes mosquito with dengue virus in a laboratory condition and examine the infection level and dynamic of ...

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

Ontleding van Midgut en speekselklieren van de Ae. aegypti Muggen

The mosquito midgut and salivary glands are key entry and exit points for pathogens such as Plasmodium parasites and Dengue viruses. This video protocol ...

Intraperitoneal Injection into Adult Zebrafish

Intraperitoneale injectie in de Adult zebravis

A convenient method for chemically treating zebrafish is to introduce the reagent into the tank water, where it will be taken up by the fish. However, ...

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

Directe levering van MAV Morpholinos Into the zebravis Otocyst door injectie en elektroporatie van invloed op binnenoor Ontwikkeling

In recent years, electroporation has become a popular technique for in vivo transfection of DNA, RNA, and morpholinos into various tissues, including the ...

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi Interferentie door dsRNA Injectie in Drosophila Embryo&#39;s

Genetic screening is one of the most powerful methods available for gaining insights into complex biological process 1. Over the years many improvements ...

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

RNAi-gemedieerde Gene Knockdown en<em&gt; In Vivo</em&gt; Urineproductie Assay in het volwassenenonderwijs Vrouw<em&gt; Aedes aegypti</em&gt; Muggen

This video protocol demonstrates an effective technique to knockdown a particular gene in an insect and conduct a novel bioassay to measure excretion ...

Measuring Peptide Translocation into Large Unilamellar Vesicles

Het meten van Peptide Translocatie in grote unilamellaire Blaasjes

There is an active interest in peptides that readily cross cell membranes without the assistance of cell membrane receptors1.  Many of these are referred ...

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

Fysiologische Recordings en RNA Sequencing van de Smaak Appendages van de Gele-koorts Mosquito<em&gt; Aedes aegypti</em

Electrophysiological recording of action potentials from sensory neurons of mosquitoes provides investigators a glimpse into the chemical perception of ...

RNAi Trigger Delivery into Anopheles gambiae Pupae

RNAi Trigger Delivery into Anopheles gambiae Pupae

RNAi Trigger delivery in<em&gt; Anopheles gambiae</em&gt; Poppen

RNA interference (RNAi), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory ...