Name: injection of dsrna into female a. aegypti mosquitos
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Injection of dsRNA into Female A. aegypti Mosquitos at JoVE.com

<ol><li> Gör mikroinjektion nålar genom att dra glas kapillärrör. Vi använder en nål avdragare från Sutter Instrument CO (modell P-2000). Nålen avdragare Programmet specifikationer: värme: 275 fil: 3 vel: 37 del: 250 PUL: 125 </li><li> Ladda en känd volym (t.ex. 1 l) vatten i barr och manuellt markera skala med en fin Sharpie. </li><li> Sätt upp en efter injektion kopp för myggen. Först klippa yttersta spetsen av av ett 1000 l pipettspetsen. Sedan skär ett hål i sidan av en kop...

injection of dsrna into female a. aegypti mosquitos

Omvänd genetiska metoder har visat sig extremt användbart för att avgöra vilka gener som underliggande motstånd mot vektor patogener i myggor. Denna video protokoll illustrerar en metod som James labbet att injicera dsRNA i kvinnliga A. aegypti myggor, som hyser den denguefeber virus. Tekniken för kalibrering injektionsnålar, manipulera injektionen setup, och injicera dsRNA i bröstkorgen illustreras.

Watch this Scientific Journal Video about Injection of dsRNA into Female A. aegypti Mosquitos at JoVE.com

Injection of dsRNA into Female A. aegypti Mosquitos

Injektion av dsRNA till Kvinna A. aegypti Mosquitos

Reverse genetic approaches have proven extremely useful for determining  which genes underly resistance to vector pathogens in mosquitoes.  This video ...

Research

JoVE Journal

Biology

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

Mikroinjektion av A. aegypti Embryon Skaffa Transgena myggor

In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue ...

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

Protocol for Dengue Infections in Mosquitoes A. aegypti and Infection Phenotype Determination

Protokoll för Dengue Infektioner i Myggor (A. aegypti) och infektion Fenotyp Fastställande

The purpose of this procedure is to infect the Aedes mosquito with dengue virus in a laboratory condition and examine the infection level and dynamic of ...

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

Dissekering av midgut och spottkörtlar från AE. aegypti Myggor

The mosquito midgut and salivary glands are key entry and exit points for pathogens such as Plasmodium parasites and Dengue viruses. This video protocol ...

Intraperitoneal Injection into Adult Zebrafish

Intraperitoneal injektion i vuxen Zebrafish

A convenient method for chemically treating zebrafish is to introduce the reagent into the tank water, where it will be taken up by the fish. However, ...

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

Direkt Leverans av MIF Morpholinos Into the Zebrafish Otocyst med injektion och elektroporation Påverkar Inner Ear utveckling

In recent years, electroporation has become a popular technique for in vivo transfection of DNA, RNA, and morpholinos into various tissues, including the ...

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi Störningar av dsRNA Injektion i Drosophila Embryon

Genetic screening is one of the most powerful methods available for gaining insights into complex biological process 1. Over the years many improvements ...

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

RNAi-mediated gene Knockdown och<em&gt; In Vivo</em&gt; Diures Analys hos vuxna kvinnliga<em&gt; Aedes aegypti</em&gt; Myggor

This video protocol demonstrates an effective technique to knockdown a particular gene in an insect and conduct a novel bioassay to measure excretion ...

Measuring Peptide Translocation into Large Unilamellar Vesicles

Mätning Peptide Translokation i stora Unilamellar Blåsor

There is an active interest in peptides that readily cross cell membranes without the assistance of cell membrane receptors1.  Many of these are referred ...

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

Fysiologiska Inspelningar och RNA sekvensering av de smak bihang till Gula-febern Mosquito<em&gt; Aedes aegypti</em

Electrophysiological recording of action potentials from sensory neurons of mosquitoes provides investigators a glimpse into the chemical perception of ...

RNAi Trigger Delivery into Anopheles gambiae Pupae

RNAi Trigger Delivery into Anopheles gambiae Pupae

RNAi Trigger Delivery in<em&gt; Anopheles gambiae</em&gt; Puppor

RNA interference (RNAi), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory ...