For popliteal draining lymph node harvest, apply mineral oil to the calf and knee on the injected side of the animal. Next, make a midline incision in the calf from the heel to the knee, and dissociate the calf musculature from the skin.
Expose the popliteal fossa. The popliteal lymph node will appear as a translucent sphere within the fossa. Then, remove the lymph node with the microsurgery curved forceps.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved