Name: the method of rodent whole embryo culture using the rotator-type bottle culture system
Uploaded: 2010-08-28T17:00:00
Description: Watch this Scientific Journal Video about The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System at JoVE.com

我们感谢肇一乘先生编辑视频录像和乐于助人的意见。我们也感谢Drs.Yuji Tsunekawa和凯驰吉崎实物录像助理。这项工作是支持KAKENHI青年科学家B和优先领域，分子由日本文部科学省的脑科学。我们承认，支持全球COE计划“全球脑科学的基础和转化研究中心”由文部科学省，日本和演化的科学和技术的核心研究（CREST）由日本科技公司从日本科学技术振兴机构（JST） 。

1。设立职业英语运动系统<ol><li>联合0.22μm的滤膜过滤，职业英语运动系统内的进口硅管 ） 。 </li><li>打开一个汽缸气体， 含 O 2（95％）和CO 2（5％）阀门。通过鼓泡瓶含有灭菌水鼓流入的气体混合物。 </li><li>调整混合气体流量50立方厘米。 </li><li>插入让出一瓶水硅管，并检查了混合气体的流量。 </li><li>在20转/分的速度旋转的鼓。 </li></ol> 2。培养基的?...

<ol>
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有成功的灭鼠职业英语运动的两个关键步骤。首先，剥离过程应该是准确的，不破坏胚胎，特别是血管。其次，程序应尽快，因为氧气和营养通过胎盘从子宫隔离后不再提供。这是对年纪较大的胚胎的关键。在E12.5大鼠职业英语运动的情况下，我们应该在30分钟内转移到培养瓶解剖胚胎。 我们已经分析了野生型和标签少量细胞用荧光染料DII或DII -标记的细胞移植在大鼠胚胎的?...

<table border="1"><tbody><tr><td> 名称 </td><td> 类型 </td><td> 公司 </td><td> 产品编号 </td><td> 评论 </td></tr><tr><td colspan="5"> 设置最多职业英语运动 </td></tr><tr><td>职业英语运动系统（10-0310） </td><td>工具</td><td>池本丽卡</td><td> 010-0310 </td><td>另外一个小的模型也可用。 </td></tr><tr><td>无孔的硅插件</td><td>工具</td><td>池本丽卡</td><td> 010-032-08 </td><td></td></tr><tr><td>混合气体气瓶</td><td>工具</td><td>日光山庄</td><td> - </td><td>含95％氧气和5％的二氧化碳。自定义的顺序。 </td></tr><tr><td>气体调节器</td><td>工具</td><td>小野制作所</td><td> WR - 11 </td><td></td></tr><tr><td> 0.22微米的过滤器Millex GS </td><td>工具</td><td> Millipore公司</td><td> SLGS033SS </td><td></td></tr><tr><td colspan="5"> 麻醉和子宫隔离 </td></tr><tr><td>大剪刀</td><td>工具</td><td> Napox </td><td> B - 7H </td><td></td></tr><tr><td>镊子</td><td>工具</td><td> Napox </td><td> A - 3 - 2 </td><td></td></tr><tr><td>处置培养皿 （90毫米× 15毫米） </td><td>工具</td><td>磐</td><td> SH90 - 15 </td><td>深型的菜是最好的解剖。 </td></tr><tr><td>异氟醚</td><td>试剂</td><td>雅培</td><td> B506 </td><td>麻醉</td></tr><tr><td>戊巴比妥钠</td><td>试剂</td><td>先灵葆雅动物保健</td><td> - </td><td>麻醉</td></tr><tr><td>台氏生理盐水</td><td>试剂</td><td> - </td><td> - </td><td>根据文献的协议。 2。保存在4 ° C。 </td></tr><tr><td>定时妊娠的SD大鼠</td><td>动物</td><td>查尔斯河实验室日本</td><td> - </td><td></td></tr><tr><td colspan="5"> 培养基的制备</td></tr><tr><td>文化玻璃瓶</td><td>工具</td><td>池本丽卡</td><td> 010-032-05 </td><td></td></tr><tr><td>处置培养瓶</td><td>工具</td><td>池本丽卡</td><td> 010-032-06 </td><td></td></tr><tr><td>硅孔插头</td><td>工具</td><td>池本丽卡</td><td> 010-032-07 </td><td></td></tr><tr><td>铝箔</td><td>工具</td><td>任何公司</td><td> - </td><td></td></tr><tr><td>灭菌袋</td><td>工具</td><td> Hogy </td><td> HM - 26 </td><td>对于培养瓶</td></tr><tr><td>灭菌袋</td><td>工具</td><td> Hogy </td><td> HM - 14A </td><td>硅插头</td></tr><tr><td> 0.45微米的过滤器Millex医管局</td><td>工具</td><td> Millipore公司</td><td> SLHA033SS </td><td></td></tr><tr><td> 50毫升锥形管</td><td>工具</td><td>碧迪</td><td> 352070 </td><td></td></tr><tr><td> 100毫升烧杯中</td><td>工具</td><td>磐</td><td> TE - 32 </td><td></td></tr><tr><td>大鼠集成电路血清</td><td>试剂</td><td>查尔斯河实验室日本</td><td> - </td><td>请参阅：邦彦森崎先生， 电话：+81-（0）45-474-9336传真：+81-（0）45-474-9340 </td></tr><tr><td> D（+）-葡萄糖</td><td>试剂</td><td>瓦科</td><td> 041-00595 </td><td></td></tr><tr><td>抗生素antimyotic液体</td><td>试剂</td><td> Gibco公司</td><td> 15240 </td><td></td></tr><tr><td colspan="5"> 解剖胚胎 </td></tr><tr><td>眼科直剪刀</td><td>工具</td><td> Napox </td><td> MB50 - 7 </td><td>切割子宫壁</td></tr><tr><td>眼科弯剪刀</td><td>工具</td><td> Napox </td><td> MB54 - 2 </td><td>切割卵黄囊</td></tr><tr><td>钳＃5 </td&gt; <td>工具</td><td>活力</td><td> T6715 </td><td></td></tr><tr><td>钳＃5F </td><td>工具</td><td>雷吉娜</td><td> T6819 </td><td></td></tr><tr><td>玻璃吸管</td><td>工具</td><td> - </td><td> - </td><td>由手使用巴斯德吸管。 </td></tr><tr><td>灭菌袋</td><td>工具</td><td> Hogy </td><td> HM - 4 </td><td>对于玻璃移液器</td></tr><tr><td>双目显微镜</td><td>工具</td><td>徕卡</td><td> Mz7s </td><td></td></tr><tr><td>光</td><td>工具</td><td>徕卡</td><td>华彩150XD </td><td></td></tr><tr><td>用油石</td><td>工具</td><td>内田洋子</td><td> 833-2000 </td><td>钳锐化</td></tr><tr><td>机械油</td><td>工具</td><td>内田洋子</td><td> 835-0000 </td><td>钳锐化</td></tr></tbody></table>

the method of rodent whole embryo culture using the rotator-type bottle culture system

全胚胎培养（WEC）技术已在1950年开发新和他的同事，和发育生物学的1应用。虽然哺乳动物胚胎的发展和增长是严重依赖对胎盘功能，职业英语运动技术使我们能够在胚胎一天（五）6.5 E12.5相应midgestation阶段，在有限的时期在文化大鼠和小鼠胚胎体外条件鼠标或在大鼠2，3，4 E8.5 - E14.5。职业英语运动，我们可以直接目标所需的领域使用细的玻璃毛细管的胚胎，因为胚胎可以在显微镜下操作。因此，灭鼠职业英语运动是非常有用的技术时，我们要研究动态postimplanted哺乳动物胚胎的发育过程。到今天为止，几种类型的职业英语运动的系统已经开发1。其中，肩，瓶型文化体系是最流行 ​​和最适合长期midgestation，即胚胎的文化，后E9.5和E11.5的小鼠和大鼠， 分别为1 。在这个视频协议，我们展示了我们的标准程序后E12.5使用了原来的旋转系统，这是由新的Cockroft 5，6设计精致模型大鼠职业英语运动，并引入职业英语运动技术的研究在哺乳动物发育的各种应用生物学。

Watch this Scientific Journal Video about The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System at JoVE.com

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

全胚胎培养技术，使我们文化的大鼠和小鼠胚胎<em&gt;体外</em&gt;在midgestation阶段对应有限的时期条件。在这个视频协议，我们将演示后，用肩瓶型文化体系E12.5大鼠全胚胎培养我们的标准程序。

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