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JoVE Journal

Biology

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The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

23.5K Views

10:04 min

August 28th, 2010

August 28th, 2010

23,528 Views

0:00

The Method of Rodent Whole Embryo Culture Using the Rotator-type Culture System

0:07

Introduction

2:06

Setting up Whole Embryo Culture System

2:52

Preparation of Culture Medium

3:53

Removal of Embryos from the Uterus

4:52

Dissection of Rat Embryos

7:27

Results and Discussion

Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.

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