Induction of Graft Versus Host Disease in Mouse Models via Allogeneic Bone Marrow and Splenic Cell Transplantation

Begin with an anesthetized recipient mouse previously irradiated to suppress the host immune system.

Obtain hematopoietic stem cells from a genetically different donor mouse to mimic allogeneic stem cell transplantation.

Remove the donor T cells using magnetic beads.

Inject this T cell-depleted suspension into the retrobulbar space of the recipient mouse.

Transplanted cells enter the bloodstream, migrate to the bone marrow vessels, and use adhesion molecules to enter the niche, initiating hematopoiesis.

Inject splenic T lymphocytes from an allogeneic donor mouse into the recipient's retrobulbar space to induce immune cross-reactivity.

These alloreactive T cells migrate to various tissues.

The T cell surface receptors bind to recipient cell antigens, recognizing them as foreign.

This triggers a cascade of signaling events within the T lymphocytes, leading to their activation.

The activated T cells trigger an inflammatory cascade characterized by the recruitment of additional immune cells that collectively damage the host tissues, leading to the development of graft-versus-host-disease.