Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs

To assess the transepithelial electrical resistance, or TEER, of the Caco-2 cells immediately after irradiation, transfer half of the Caco-2 cell culture inserts into a new plate with 3 milliliters of fresh complete medium in each well, and half of the insert cultures into a new plate seeded with 2 x 10⁶ PBMC per 3 milliliters of complete medium per well. Then, place a TEER chopstick electrode into the cell culture insert every hour for the first six hours, and then every three hours until 48 hours after irradiation.