Preparation of a Nanoemulsion Adjuvant Vaccine

Begin by mixing 1 milligram of monophosphoryl lipid A with 100 microliters of DMSO. Vortex for five minutes and leave it to dissolve for four hours at room temperature. Quantitatively add Tween 80 and I-OVA. Mix the components and add squalene.

Next, add 100 microliters of MPLA solution to the prepared mixture. To prepare the nanoemulsion vaccine, add the mixed solution to water droplets at approximately 70% of the total volume and stir gently to obtain a transparent and easily flowing mixture.