An Assay To Evaluate the Anticancer Effects of Lactobacillus Cell-Free Supernatant

For LCFS treatment, serially dilute the prepared thawed LCFS stock solution in fresh growth medium to 25%, 12.5, and 6% concentrations and use a 200-microliter pipette to carefully remove as much supernatant as possible from each well of the plated colorectal cancer cell line cultures.

Then gently add 200 microliters of the LCFS-supplemented growth medium in triplicate to each set of cancer cells, and return the plate to the incubator for an additional 24 to 48 hours.