Name: optic nerve transection: a model of adult neuron apoptosis in the central nervous system
Uploaded: 2011-05-12T12:00:00
Description: Watch this Scientific Journal Video about Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System at JoVE.com

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1. Cerrahi Teknik <ol><li> Aseptik teknik kullanılarak deneyler ve özel kurum hayvan kullanımını protokolleri takip yapılmalıdır. Canlı doku ile temas eden alet ve malzemeler (çözümleri, test maddeler, izleyiciler, iğne, vb), hayvan refahı ve çalışma üzerindeki olası olumsuz etkileri üzerinde enfeksiyon ve olumsuz etkilerini önlemek için steril olmalıdır. </li></ol> 2. Anestezi <ol><li> Sıçanlar bir veteriner izofluran buharlaştırıcı sistemi kullanılarak anestez...

     

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D., Gauldie, J., Ball, A. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+adenoviral-mediated+gene+transfer+of+interleukin-10,+interleukin-4,+and+transforming+growth+factor-beta+on+the+survival+of+axotomized+retinal+ganglion+cells.">Effects of adenoviral-mediated gene transfer of interleukin-10, interleukin-4, and transforming growth factor-beta on the survival of axotomized retinal ganglion cells.</a> Neuroscience. 125, 903-920 (2004).</li><li>Berkelaar, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Axotomy+results+in+delayed+death+and+apoptosis+of+retinal+ganglion+cells+in+adult+rats.">Axotomy results in delayed death and apoptosis of retinal ganglion cells in adult rats.</a> J Neurosci. 14, 4368-4374 (1994).</li><li>Villegas-Perez, M. 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Bu cerrahi prosedürün çok varyasyon vardır ve bu protokol adımları birkaç gerekli değildir. Sadece sinir erişmek için optik sinir örten kasları geri çekmek için gerekli. Ancak, sinir çevresinde çok sınırlı bir çalışma alanı bu sonuçları kesisi kritik son aşamaya daha zor hale. Bazı durumlarda, optik sinir güdük hücreleri ve tüm ekstraoküler kasları ve lakrimal bezi bu durumda faydalıdır geri çekilmeden sağladığı artan erişim alanı transfect arzu edilir. <p class="jove_conten...

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<td>&nbsp;</td>
<td>Stoelting, Kopf, WPI</td>
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<td>Rat Gas Mask</td>
<td>&nbsp;</td>
<td>Stoelting, Kopf, WPI</td>
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<td>Anesthesia System</td>
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<td>VetEquip</td>
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<td>Isoflurane (PrAErrane)</td>
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<td>Baxter Corp</td>
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<td>Surgical Microscope</td>
<td>&nbsp;</td>
<td>WPI, Zeiss, Leica</td>
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<td>Fluorogold -(Hydroxystilbamidine bis(methanesulfonate)</td>
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<td>Fine tip Dumont forceps</td>
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<td>Fine Science Tools</td>
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<td>Micro surgical hook</td>
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<td>Cauterizer</td>
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<td>Fine Science Tools</td>
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optic nerve transection: a model of adult neuron apoptosis in the central nervous system

Retina ganglion hücrelerinin (RGCs) MSS nöronlar olduğunu retina optik sinir yoluyla beyne görsel bilgi çıktı. Optik sinir göz yörüngesinin içinde erişilebilir ve tüm RGC nüfus aksonlar tamamen kesme, (axotomized) transeksiyon olabilir. Optik sinir transeksiyonu yetişkin MSS 1-4 apoptotik nöron hücre ölümü tekrarlanabilir bir model. Bu model özellikle çekici, göz vitreus kamara göz içi enjeksiyonları ile deneysel manipülasyonlar izin retina ilaç dağıtım için bir kapsül gibi davranır çünkü. Vitreus sıvı ile kimyasal difüzyon tüm RGC nüfus üzerine hareket etmelerini sağlar. Ayrıca, RGCs seçici hedef, üstün colliculus 8 içine optik sinir 5-7 veya enjekte vektörler kesme sonuna kadar kısa müdahale RNA&#39;lar (siRNA), plazmid ya da viral vektörlerin uygulayarak transfekte olabilir. Bu araştırmacılar, diğer seyirci nöron veya glia çevreleyen karıştırıcı etkisi olmadan istenilen nöronal nüfus apoptotik mekanizmalar çalışma sağlar. Ek bir avantaj, kolaylık ve doğruluk hücre sağkalım yaralanma sonrası sayısal olabilir. Retinanın düz, katmanlı bir dokudur ve RGCs en içteki tabaka, ganglion hücre tabakası lokalize. RGCs, hayatta kalma, bir floresan tracer (% 3 Fluorogold), axotomy zaman optik sinir kesme sonuna kadar uygulayarak, ya da İzleyicinin üstün colliculus (RGC hedef) bir hafta önce içine enjekte edilerek zaman içinde takip edilebilir axotomy. Tracer retrograd tüm RGC nüfus etiketleme, taşınır. Ganglion hücre tabakası bir tek tabaka (kalın bir hücre) olduğundan, RGC yoğunlukları stereology için gerek kalmadan düz monte edilmiş doku nicel olabilir. Optik sinir transeksiyonu 14 postaxotomy 9-11 gün içinde% 90 yaralı RGCs apoptotik ölüme yol açar. RGC apoptozis, hücre ölümü hücrelerin hızla dejenere sonra 3-4 gün postaxotomy, gecikmiş sayede ders karakteristik bir zaman vardır. Bu deneysel manipülasyonlar apoptozis dahil yollar yöneltilen bir zaman penceresi sağlar.

Watch this Scientific Journal Video about Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System at JoVE.com

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Optik Sinir transeksiyonu yetişkin MSS yaralanma yaygın olarak kullanılan bir model. Retina ganglion hücrelerinin yüzde doksanı (RGCs) olan aksonlar tamamen axotomy sonra 14 gün içinde ölürler (axotomy) transeksiyon. Bu model, deneysel manipülasyonlar kolayca mükellef ve yüksek tekrarlanabilir.

Optik Sinir transeksiyonu: Santral Sinir Sistemi Yetişkin Nöron Apoptoz Bir Model

Retinal ganglion cells (RGCs) are CNS neurons that output visual information from the retina to the brain, via the optic nerve. 
The optic nerve can be ...

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Neuroscience

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

Memeli MSS Optik Sinir transeksiyonu sonra Deneysel İşlemler Yöntemleri

Retinal ganglion cells (RGCs) are CNS neurons that output visual information from the retina to the brain, via the optic nerve. The optic nerve can be ...

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Fareler ve Merkezi Sinir Sisteminde Kök Hücre İmplantasyonu Multimodal Görüntüleme

During the past decade, stem cell transplantation has gained increasing interest as primary or secondary therapeutic modality for a variety of diseases, ...

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Merkezi sinir sisteminin transducing Hücreleri için lentiviral Vektörler Üretimi

Efficient gene delivery in the central nervous system (CNS) is important in studying gene functions, modeling neurological diseases and developing ...

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Merkez Sinir Sistemi Tek Hücreler Etiketleme<em&gt; Drosophila melanogaster</em

In this  article we describe how to individually label neurons in the embryonic CNS of Drosophila melanogaster by juxtacellular injection of the ...

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Santral Sinir Sistemi Onarım İnceleyecek Bir Sakatlık Paradigma<em&gt; Drosophila</em

An experimental method has been developed to investigate the cellular responses to central nervous system (CNS) injury using the fruit-fly Drosophila. ...

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Kemirgen Merkezi Sinir Sistemi İlaçların doğrudan Intraventriküler teslim

Due to an inability to cross the blood brain barrier, certain drugs need to be directly delivered into the central nervous system (CNS). Our lab focuses ...

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

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Polyribosome Fraksiyonu tarafından Santral Sinir Sistemi Translatome in vivo sorguluyor

Multiple processes are involved in gene expression including transcription, translation and stability of mRNAs and proteins. Each of these steps are ...

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

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Kemirgen Santral Sinir Sistemi vivo optogenetic Stimülasyon içinde

The ability to probe defined neural circuits in awake, freely-moving animals with cell-type specificity, spatial precision, and high temporal resolution ...

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

Deneysel Optik Sinir demyelinizasyon bir Rat Modelinde Görsel Uyarılmış Potansiyel Kayıt

The visual evoked potential (VEP) recording is widely used in clinical practice to assess the severity of optic neuritis in its acute phase, and to ...

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Pasif kullanımı Netlik Fare Merkezi Sinir Sistemi Optik Takas

Traditionally, tissue visualization has required that the tissue of interest be serially sectioned and imaged, subjecting each tissue section to unique ...