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Differentiation of Spinal Cord Neural Progenitor Cells into Motor Neurons


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If the neural progenitor cell cultures were frozen, thaw them. After medium exchange and PBS rinsing as described in the manuscript, change the medium with a motor neuron differentiation medium containing 0.02 micromolar cytosine arabinoside.

Then, incubate the cells for 48 hours at 37 degrees Celsius and 5% carbon dioxide when glial-committed progenitors emerge as single proliferating flat cells under post-mitotic motor neuron progenitors aggregated in cell clusters. Later, perform medium exchange as indicated in the manuscript.

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