Differentiation of Spinal Cord Neural Progenitor Cells into Motor Neurons

If the neural progenitor cell cultures were frozen, thaw them. After medium exchange and PBS rinsing as described in the manuscript, change the medium with a motor neuron differentiation medium containing 0.02 micromolar cytosine arabinoside.

Then, incubate the cells for 48 hours at 37 degrees Celsius and 5% carbon dioxide when glial-committed progenitors emerge as single proliferating flat cells under post-mitotic motor neuron progenitors aggregated in cell clusters. Later, perform medium exchange as indicated in the manuscript.