Name: methods for experimental manipulations after optic nerve transection in the mammalian cns
Uploaded: 2011-05-12T14:03:00
Description: Watch this Scientific Journal Video about Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS at JoVE.com

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1。手術手技<ol><li>実験は無菌操作を使用して、特定の機関の動物使用のプロトコールに従って実施されるべきである。生体組織と接触する楽器と材料（ソリューション、テスト物質、トレーサー、針、など）動物福祉と研究上の潜在的な負の影響に感染して悪影響を防ぐために無菌でなければならない。 </li></ol> 2。麻酔<ol><li>ラットは、獣医イソフルラン気化器のシス?...

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視神経切断は、成体中枢神経系ニューロンのアポトーシスの高度に再現可能なモデルです。この原稿で実証実験操作は、損傷後のRGCアポトーシスのメカニズムの研究を可能にする。 眼内注射は、網膜の全世界をターゲットするのに便利です。それはガラスピペットの先端にレンズを傷つけないよう重要であるとして、この手順では、いくつかの練習が必要です。レンズ?...

A correction to the article Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS was made.

Erratum: Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

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<td>Stereotaxic Frame</td>
<td>&nbsp;</td>
<td>Stoelting, Kopf, WPI</td>
<td>&nbsp;</td>
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<td>Rat Gas Mask</td>
<td>&nbsp;</td>
<td>Stoelting, Kopf, WPI</td>
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<td>&nbsp;</td>
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<td>Anesthesia System</td>
<td>&nbsp;</td>
<td>VetEquip</td>
<td>901806</td>
<td>&nbsp;</td>
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<td>Isoflurane (PrAErrane)</td>
<td>&nbsp;</td>
<td>Baxter Corp</td>
<td>DIN 02225875</td>
<td>&nbsp;</td>
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<td>Surgical Microscope</td>
<td>&nbsp;</td>
<td>WPI, Zeiss, Leica</td>
<td>&nbsp;</td>
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<td>Alcaine Eye Drops</td>
<td>&nbsp;</td>
<td>Alcon</td>
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<td>Tears Naturale P.M.</td>
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<td>Alcon</td>
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<td>Fine tip Dumont forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11252-00</td>
<td>&nbsp;</td>
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<td>10 &mu;l Hamilton Syringe (1701RN; 26s/2&#x201D;/2)</td>
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<td>Hamilton Syringe Co.</td>
<td>80030</td>
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<td>1/16 inch Compression Fittings</td>
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<td>Hamilton Syringe Co.</td>
<td>55751-01</td>
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<td>1/16 inch OD, 0.010 inch ID, PEEK Tubing</td>
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<td>Supelco, Bellefonte, PA</td>
<td>Z226661</td>
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<td>Dual RN Glass Coupler</td>
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<td>Hamilton Syringe Co.</td>
<td>55752-01</td>
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<td>Mineral Oil Priming Kit: includes syringe, needles, rubber septa</td>
<td>&nbsp;</td>
<td>Hamilton Syringe Co.</td>
<td>PRMKIT</td>
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methods for experimental manipulations after optic nerve transection in the mammalian cns

網膜神経節細胞は（RGCs）視神経を経由して、網膜から脳にその出力を視覚情報CNSニューロンです。視神経は、全体RGC​​人口の軸索を切断、目と完全にtransected（axotomized）の軌道内でアクセスすることができます。視神経切断は、成体中枢神経系1-4のアポトーシス神経細胞死の再現可能なモデルです。眼の硝子体腔は眼内注射を介して実験操作を可能にして、網膜への薬物送達のためのカプセルとして機能するので、このモデルは特に魅力的です。硝子体液を介して化学物質の拡散は、彼らが全体のRGCの人口に基づいて行動することを保証します。ウイルスベクター、プラスミドまたは短い干渉RNAは（siRNAが）も5-12を網膜細胞に感染またはトランスフェクトするために硝子体腔に配信することができます。アデノ随伴ウイルス（AAV）ベクターの高い親和性は、注射部位6、7、13-15近くの細胞の90％に近い感染率で、ターゲットRGCsに有益です。また、RGCsが選択的に標的上丘10に視神経16から19または注入ベクトルの切り口にしたsiRNA、プラスミド、またはウイルスベクターを適用することにより、トランスフェクションすることができる。これは、研究者は他のバイスタンダーの神経細胞または周囲のグリア細胞上で交絡の影響なしに負傷した神経細胞集団のアポトーシスのメカニズムを研究することができます。 RGCのアポトーシスは、細胞死、細胞が急速に退化した後、3〜4日のpostaxotomyを遅延さにより時間経過特性を持っています。これは、アポトーシスに関与する経路に対して誘導実験操作のためのウィンドウが用意されています。直接transected視神経の切り株からRGCsをターゲットの操作はすぐに神経を切断した後、軸索切断時に実行されます。これとは対照的に、物質が眼内経路を介して配信されるとき、それらはaxotomized RGCsのアポトーシスの開始の前に、手術前または手術後3日以内に注入することができる。現在の記事では、視神経切断後の実験操作にはいくつかの方法を示しています。

Watch this Scientific Journal Video about Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS at JoVE.com

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

視神経切断は、成体中枢神経系の損傷の広く使用されているモデルです。このモデルは、世界的に網膜をターゲットにするか、直接網膜神経節細胞の損傷した神経細胞集団を対象に実験操作の数を実行するための理想的です。

哺乳類の中枢神経系における視神経切断後の実験操作のための方法

Retinal ganglion cells (RGCs) are CNS neurons that output visual information from the retina to the brain, via the optic nerve. The optic nerve can be ...

Research

JoVE Journal

Neuroscience

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

視神経切断：中枢神経系における成人ニューロンのアポトーシスのモデル

Retinal ganglion cells (RGCs) are CNS neurons that output visual information from the retina to the brain, via the optic nerve. 
The optic nerve can be ...

神経科学

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

網膜神経節細胞の生存を研究するために視神経挫滅モデルマウス

Injury to the optic nerve can lead to axonal degeneration, followed by a gradual death of retinal ganglion cells (RGCs), which results in irreversible ...

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

末梢神経損傷後の機能回復を評価する嗅神経鞘細胞の移植

Olfactory ensheathing cells (OECs) are neural crest cells which allow growth and regrowth of the primary olfactory neurons. Indeed, the primary olfactory ...

Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish

モーター神経切断とライブゼブラフィッシュにおけるグリア細胞の挙動のタイムラプスイメージング

The  nervous system is often described as a hard-wired component of the body even  though it is a considerably fluid organ system that reacts to external ...

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

グルタミン酸とGABAの高速マイクロイオン導入：シナプス統合を調査するために便利なツール

One of the fundamental interests in neuroscience is to understand the integration of excitatory and inhibitory inputs along the very complex structure of ...

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

マウスにおける造影MRIによる視神経線維の整合性in vivoイメージング

The rodent visual system encompasses retinal ganglion cells and their axons that form the optic nerve to enter thalamic and midbrain centers, and ...

Spinal Cord Transection in the Larval Zebrafish

幼虫のゼブラフィッシュにおける脊髄切断

Mammals fail in sensory and motor recovery following spinal cord injury due to lack of axonal regrowth below the level of injury as well as an inability ...

Minimally-invasive Technique for Injection into Rat Optic Nerve

ラット視神経への注入のための低侵襲テクニック

The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central ...

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

実験視神経の脱髄のラットモデルにおける視覚誘発電位の記録

The visual evoked potential (VEP) recording is widely used in clinical practice to assess the severity of optic neuritis in its acute phase, and to ...

Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

組織学的分析のための豚CNSの露出：斬首、スカルオープニングのためのマニュアル、および脳の取り外し

Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to ...