Co-culturing of a Dorsal Root Ganglion Explant with Schwann Cells for Neuron Myelination

Take a four-well plate containing 190 microliters of DRG growth medium in each well, and carefully transfer a single DRG into the center of each well using fine forceps and a spatula. Then, place the DRG explant cultures in the incubator at 37 degrees Celsius and 5% carbon dioxide. The next day, carefully add 50 microliters of the DRG growth medium to each well, and observe DRG explant adherence and axon outgrowth using a microscope daily. For co-culturing on day three of the DRG explant culture, carefully replaced the DRG growth medium with 250 microliters of the co-culture medium containing 30,000 Schwann cells per well.