Isolation of Neurons from Caenorhabditis elegans via Fluorescence-Activated Cell Sorting

Centrifuge cells from the isolated cell suspension at 10,000 times g and at 4 degrees Celsius for 5 minutes. Discard the supernatant and resuspend the pelleted cells in L-15-supplemented medium to a cell density of no more than 6 million cells per milliliter to avoid overloading the flow cytometer. Then, sort cells by GFP-positive expression using a flow cytometer capable of sorting samples.

GFP-negative cells can be used as a control for non-cell-type-specific analysis. After this, plate the isolated cells into the wells of a sterile six-well plate. Place the plate into a plastic container. Add a damp wipe or soft tissue paper to add moisture to the cells and incubate at 20 degrees Celsius.