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DOI :
10.3791/2270-v
November 16th, 2010
Chapters
0:00
Title
2:16
Preparing and Fixing Dissociated Purified Rat Retinal Ganglion Cells
7:04
Image Analysis and Co-localized Puncta Quantification
1:17
Introduction
5:33
Imaging
9:18
Preparing and Fixing Brain Sections
12:39
Imaging of Tissue Sections
17:15
Conclusion
13:34
Representative Imaging and Synapse Assay Results
该协议的细节,如何量化,在分离神经元的文化和突触数量在脑切片,用免疫细胞化学。使用车厢的特异性抗体,我们的标签突触前终端以及突触后专业化的网站。我们定义这些标记所产生的信号的共定位点之间的突触。
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