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JoVE Journal

Neuroscience

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Membrane Potentials, Synaptic Responses, Neuronal Circuitry, Neuromodulation and Muscle Histology Using the Crayfish: Student Laboratory Exercises

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16:16 min

January 18th, 2011

January 18th, 2011

58,285 Views

0:00

Title

1:30

Surgical Procedure to Expose the Abdominal Extensor Muscles

3:35

Intracellular Recording from the Abdominal Extensors Muscle Fibers

5:50

Results: Effect of K+ Concentration on Intracellular Recordings of Extensor Muscle Fibers

6:42

Neuroanatomical Observations of Abdominal Extensor Muscles

8:03

Recording Synaptic Responses in Abdominal Extensor Muscles

10:00

Results: Comparing Synaptic Responses of DEL and SEL Muscles

11:19

Intracellular Recording of EPSPs and IPSPs from Superficial Flexor Muscles

12:35

Results: EPSPs of Superficial Flexor Muscle Fibers

12:46

Extracellular Recording from Motor Neurons of the Superficial

14:27

Results: Motor Neuron Response to Sensory Stimulation and Serotonin

15:30

Conclusion

The experiments demonstrate an easy approach for students to gain experience in examining muscle structure, synaptic responses, the effects of ion gradients and permeability on membrane potentials. Also, a sensory-CNS-motor-muscle circuit is presented to show a means to test effects of compounds on a neuronal circuit.

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