Name: muscle receptor organs in the crayfish abdomen: a student laboratory exercise in proprioception
Uploaded: 2010-11-18T17:00:00
Duration: 10 min 50 s
Description: Watch this Scientific Journal Video about Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception at JoVE.com

Ondersteund door de Universiteit van Kentucky, Departement Biologie, Bureau van Undergraduate Studies en College of Arts &amp; Sciences.

<ol>
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De gegevens die in de bijbehorende film en de tekst hebben belangrijke stappen om de activiteit in de MRO van de rivierkreeft voldoende record in situ. Een doel van ons verslag is om het bewustzijn te verhogen van het potentieel voor dit preparaat in studentenhuizen onderzoek laboratoria uitgevoerd om fundamentele concepten te onderwijzen in de sensorische fysiologie. De voorbereidingen zijn zeer robuust van de levensvatbaarheid terwijl badend in een minimale zoutoplossing. De motor controle op de MRO spieren is geïdentificeerd, maar de regelgeving in de transmissie en het potentieel van synaptische plasticiteit en effectiviteit van transmissie voor de exciterende en inhiberende neuronen blijft een open ruimte voor onderzoek (Elekes en Florey, 1987a, b; Florey en Florey, 1955; Kuffler, 1954; Kuffler en Eyzaguirre, 1955). Dit preparaat kan worden gebruikt om een ​​aantal experimentele omstandigheden en het natuurlijke verspreidingsgebied van voortbewegen in het dier te onderzoeken om een ​​beter inzicht in de biologie van de MRO voor primaire onderzoek en demonstratieve doeleinden. De biofysische eigenschappen van deze sensorische neuronen is voor een deel aan bod gekomen in de aard van de aanpassing in de neurale activiteit met een onderhouden stimulus (Brown et al., 1978; Edwards et al., 1981;. Purali, 1997; Rydqvist en Purali, 1991; Rydqvist en Swerup, 1991). Echter, slechts een paar meldingen adres neuromodulatie op deze zintuiglijke receptoren en de bijbehorende spiervezels (Cooper et al., 2003;. Pasztor en Macmillan, 1990). De rapporten gaan met slechts een paar van de vele verbindingen die bekend is dat ze aanwezig zijn in de hemolymfe. Veel modulatoren en cocktails van modulators nog moeten worden onderzocht op de MRO complex (spieren en neuronen). Pasztor en Macmillan (1990) heeft onderzocht van de neuromodulatoren 5-HT en octopamine op de activiteit van basis-herfinancieringstransacties tussen de verschillende soorten en schaaldieren opgemerkt dat er soorten verschillen. Ze hadden niet in detail in op de lange termijn invloeden van deze neuromodulatoren, noch de effecten op de activiteit op verschillende statische posities van de MRO. Dit soort van voorbereiding kan helpen bij het ​​begrijpen van de basis van de zintuiglijke waarneming en de regulering van neurale verwerking die belangrijk is in revalidatie en disease management voor mensen met een motorische eenheid abnormities (Patel et al., 2009;. Rabin et al., 2009;. Marino et al. ., 2010). De verschillende soorten van input en het afvuren van patronen voor het toezicht op de gezamenlijke bewegingen in een toegankelijke ongewervelde preparaten kunnen worden gebruikt in de robotica / prothese (Macmillan en Patullo, 2001). Er zijn nog veel vragen te wachten antwoorden in dit preparaat dat nuttig kan zijn in een aantal manieren.

<ol>
<li><a href="http://www.adinstruments.com/products/hardware/corporate/product/ML856/" target="_blank">PowerLab 26T (AD Instruments)</a> </li>
<li><a href="http://www.adinstruments.com/products/software/research/LabChart-Software/" target="_blank">LabChart 7 (ADI Instruments, Colorado Springs, CO, USA)</a> </li>
</ol>

muscle receptor organs in the crayfish abdomen: a student laboratory exercise in proprioception

Het primaire doel van dit experiment is om aan te tonen primaire sensorische neuronen overbrengen van informatie van de gezamenlijke bewegingen en posities als proprioceptieve informatie voor een dier. Een bijkomende doelstelling van dit experiment is om de anatomie van het preparaat te leren door kleuring, dissectie en bekijken van neuronen en sensorische structuren onder een dissectie microscoop. Dit wordt uitgevoerd met behulp van elementaire neurofysiologische apparatuur om de elektrische activiteit record uit een gezamenlijke receptor orgel en vlekken technieken. De spier-receptor orgel (MRO)-systeem in de rivierkreeft is analoog aan de intrafusal spier as in zoogdieren, die helpt bij het dienen als een vergelijkend model dat is beter toegankelijk voor elektrofysiologische opnames. Bovendien zijn deze herkenbaar zijn sensorische neuronen bij de voorbereidingen. De voorbereiding is haalbaar in een minimale zoutoplossing voor de uren die vatbaar is voor de student praktische oefeningen. De MRO is ook gevoelig voor neuromodulatie, die intrigerende vragen in de sites van de modulerende werking en de integratie van dynamische signalen van bewegingen en een statische positie, samen met een winst die kan worden veranderd in het systeem bevordert.

Watch this Scientific Journal Video about Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception at JoVE.com

Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception

Het primaire doel van dit experiment is om te begrijpen hoe de primaire sensorische neuronen informatie van gezamenlijke bewegingen en posities als proprioceptieve informatie voor een dier over te brengen. Een bijkomende doelstelling van dit rapport aanwezig is de anatomie van de voorbereiding door dissectie en het bekijken van neuronen in een dissectie microscoop.

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Fysiologische Experimenteren met de rivierkreeftjes einddarm: een student practicum

The purpose of the report is to describe dissection techniques for preparing the crayfish hindgut and to demonstrate how to make physiological recordings ...

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Imaging pHluorin-tagged Receptor invoegen in op het plasmamembraan in primaire gekweekte muis neuronen

A better understanding of the mechanisms governing receptor trafficking between the plasma membrane (PM) and intracellular compartments requires an ...

Proprioception and Tension Receptors in Crab Limbs: Student Laboratory Exercises

Proprioceptie en Tension Receptoren in Crab Ledematen: Student Laboratorium Oefeningen

The primary purpose of these procedures is to demonstrate for teaching and research purposes how to record the activity of living primary sensory neurons ...

Measuring Oral Fatty Acid Thresholds, Fat Perception, Fatty Food Liking, and Papillae Density in Humans

Meten Oral Fatty Acid Drempels, Fat Perception, vet voedsel Liking en papillen Dichtheid bij de mens

Emerging evidence from a number of laboratories indicates that humans have the ability to identify fatty acids in the oral cavity, presumably via fatty ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Hele Mount immunolabeling van olfactorische receptor neuronen in de<em&gt; Drosophila</em&gt; Antenne

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Een<em&gt; In Vitro</em&gt; Adult Mouse spier-zenuw Voorbereiding voor het bestuderen van de Firing Eigenschappen van Muscle afferenten

Muscle sensory neurons innervating muscle spindles and Golgi tendon organs encode length and force changes essential to proprioception. Additional ...