Name: muscle receptor organs in the crayfish abdomen: a student laboratory exercise in proprioception
Uploaded: 2010-11-18T17:00:00
Duration: 10 min 50 s
Description: Watch this Scientific Journal Video about Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception at JoVE.com

Supportato da University of Kentucky, Dipartimento di Biologia, Ufficio di studi universitari e il College of Arts &amp; Sciences.

<ol>
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Nessun conflitto di interessi dichiarati.

I dati forniti nel film associati e testo hanno fornito passaggi chiave al fine di registrare l&#39;attività sufficientemente nella ORP del gambero in situ. Uno degli obiettivi della nostra relazione è quello di aumentare la consapevolezza del potenziale di questa preparazione dello studente in corsa laboratori investigativi per insegnare concetti fondamentali nella fisiologia sensoriale. I preparativi sono molto robuste della redditività pur essendo immersi in una soluzione salina minimo. Il controllo del motore sui muscoli MRO è stato identificato, ma il regolamento in trasmissione e le potenzialità della plasticità sinaptica e l&#39;efficacia di trasmissione per i neuroni eccitatori e inibitori rimane uno spazio aperto per le indagini (Elekes e Florey, 1987a, b; Florey e Florey, 1955; Kuffler, 1954; Kuffler e Eyzaguirre, 1955). Questa preparazione può essere utilizzato per indagare su una serie di condizioni sperimentali come pure l&#39;area naturale di locomozione all&#39;interno l&#39;animale per comprendere meglio la biologia del MRO per la ricerca primaria e dimostrative. Le proprietà biofisiche di questi neuroni sensoriali è in parte stato affrontato nella loro natura di adattamento nelle attività neurale con uno stimolo mantenuto (Brown et al, 1978; Edwards et al, 1981;. Purali, 1997; Rydqvist e Purali, 1991; Rydqvist e Swerup, 1991). Tuttavia, solo pochi neuromodulazione indirizzo rapporti su questi recettori sensoriali e fibre muscolari associati (Cooper et al, 2003;. Pasztor e Macmillan, 1990). Le relazioni trattano solo alcuni dei molti composti che sono noti per essere presenti nella emolinfa. Modulatori molti e cocktail di modulatori rimangono da esaminare sul complesso MRO (muscoli e neuroni). Pasztor e Macmillan (1990) ha esaminato i neuromodulatori 5-HT e octopamina sull&#39;attività di ORP tra le varie specie di crostacei e ha notato che ci sono differenze di specie. Essi non ha esaminato nel dettaglio le influenze a lungo termine di questi neuromodulatori, né gli effetti sulle attività in diverse posizioni statiche del MRO. Questo tipo di preparazione può aiutare a comprendere le basi della percezione sensoriale e la regolazione dei processi neurali che è importante nella riabilitazione e gestione della malattia per l&#39;uomo con anormalità blocco motore (Patel et al, 2009;. Rabin et al, 2009;. Marino et al ., 2010). I vari tipi di input e modelli di cottura per il controllo dei movimenti congiunta accessibile preparati invertebrati possono essere usati in robotica / protesi (Macmillan e Patullo, 2001). Ci sono ancora molte domande in attesa di risposte in questa preparazione che può essere utile in molti modi.

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<li><a href="http://www.adinstruments.com/products/software/research/LabChart-Software/" target="_blank">LabChart 7 (ADI Instruments, Colorado Springs, CO, USA)</a> </li>
</ol>

muscle receptor organs in the crayfish abdomen: a student laboratory exercise in proprioception

Lo scopo principale di questo esperimento è quello di dimostrare primario informazioni sensoriali neuroni trasporto di movimenti e posizioni comuni, come informazioni propriocettive per un animale. Un ulteriore obiettivo di questo esperimento è quello di imparare l&#39;anatomia della preparazione di colorazione, la dissezione e la visualizzazione dei neuroni sensoriali e le strutture sotto un microscopio da dissezione. Questa operazione viene eseguita utilizzando attrezzature di base neurofisiologica per registrare l&#39;attività elettrica di un organo recettore comune e tecniche di colorazione. Il recettore muscolare organo (MRO) sistema il gambero di fiume è analogo al fuso muscolare intrafusali nei mammiferi, il che aiuta a servire come un modello comparativo che è più facilmente accessibile per le registrazioni elettrofisiologiche. In aggiunta, questi sono identificabili neuroni sensoriali tra preparativi. La preparazione è vitale in una soluzione salina minimo di ore che è suscettibile di esercitazioni di laboratorio degli studenti. Il MRO è anche suscettibile di neuromodulazione che incoraggia domande intriganti nei siti di azione di modulazione e l&#39;integrazione dei segnali dinamici di movimenti e posizione statica con un guadagno che può essere cambiato nel nostro sistema.

Watch this Scientific Journal Video about Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception at JoVE.com

Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception

Lo scopo principale di questo esperimento è quello di capire in che modo i neuroni sensoriali primari trasmettere informazioni di movimenti e posizioni comuni, come informazioni propriocettive per un animale. Un ulteriore obiettivo di questa relazione è presente l&#39;anatomia della preparazione di dissezione e la visualizzazione dei neuroni sotto un microscopio da dissezione.

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La sperimentazione con la fisiologica hindgut gamberi: un esercizio di laboratorio per studenti

The purpose of the report is to describe dissection techniques for preparing the crayfish hindgut and to demonstrate how to make physiological recordings ...

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Imaging pHluorin-tagged inserimento recettore alla membrana plasmatica nella Primaria neuroni mouse coltivate

A better understanding of the mechanisms governing receptor trafficking between the plasma membrane (PM) and intracellular compartments requires an ...

Proprioception and Tension Receptors in Crab Limbs: Student Laboratory Exercises

Propriocezione e la tensione recettori in Crab Arti: Esercizi di laboratorio per studenti

The primary purpose of these procedures is to demonstrate for teaching and research purposes how to record the activity of living primary sensory neurons ...

Measuring Oral Fatty Acid Thresholds, Fat Perception, Fatty Food Liking, and Papillae Density in Humans

Misurazione orale acidi grassi Soglie, Fat Percezione, grasso alimentare Simpatia, e Papille densità di esseri umani

Emerging evidence from a number of laboratories indicates that humans have the ability to identify fatty acids in the oral cavity, presumably via fatty ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Tutta Monte immunomarcatura di recettore olfattivo neuroni nel<em&gt; Drosophila</em&gt; Antenna

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Un<em&gt; In Vitro</emPreparazione muscolare-nervosa&gt; Adult mouse per studiare le proprietà di cottura delle afferenze muscolari

Muscle sensory neurons innervating muscle spindles and Golgi tendon organs encode length and force changes essential to proprioception. Additional ...