Name: muscle receptor organs in the crayfish abdomen: a student laboratory exercise in proprioception
Uploaded: 2010-11-18T17:00:00
Duration: 10 min 50 s
Description: Watch this Scientific Journal Video about Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception at JoVE.com

Apoiada pela Universidade de Kentucky, Departamento de Biologia, Instituto de Estudos de Graduação e Faculdade de Artes e Ciências.

<ol>
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Não há conflitos de interesse declarados.

Os detalhes fornecidos no filme e texto associados deram passos fundamentais para suficientemente registrar a atividade no MRO do crustáceo in situ. Um dos objetivos do nosso relatório é aumentar a consciência do potencial de elaboração no aluno executar laboratórios de investigação para ensinar conceitos fundamentais na fisiologia sensorial. Os preparativos são muito robustas da viabilidade ao ser banhado em uma solução salina mínimo. O controle motor dos músculos MRO foi identificado, mas a regulação da transmissão e do potencial de plasticidade sináptica, bem como a eficácia da transmissão para os neurônios excitatórios e inibitórios continua a ser um espaço aberto para a investigação (Elekes e Florey, 1987a, b; Florey e Florey, 1955; Kuffler, 1954; Kuffler e Eyzaguirre, 1955). Esta preparação pode ser usado para investigar uma série de condições experimentais, bem como a área natural de locomoção dentro do animal para melhor compreender a biologia do MRO para pesquisa primária, bem como fins de demonstração. As propriedades biofísicas destes neurônios sensoriais foi em parte abordado em sua natureza de adaptação na atividade neural com um estímulo mantido (Brown et al, 1978; Edwards et al, 1981;. Purali, 1997; Rydqvist e Purali, 1991; Rydqvist e Swerup, 1991). No entanto, apenas um endereço de neuromodulação alguns relatórios sobre esses receptores sensoriais e fibras musculares associados (Cooper et al, 2003;. Pasztor e Macmillan, 1990). Os relatórios de lidar com apenas alguns dos muitos compostos que são conhecidos para estar presente na hemolinfa. Moduladores muitos e cocktails de moduladores ainda precisam ser examinados no complexo MRO (músculos e neurônios). Pasztor e Macmillan (1990) fez examinar os neuromoduladores 5-HT e octopamine sobre a actividade da OPR entre várias espécies de crustáceos e observou que existem diferenças de espécies. Eles não examinar em detalhe as influências de longo prazo desses neuromoduladores nem os efeitos sobre a atividade em diferentes posições estáticas da MRO. Este tipo de preparação pode ajudar na compreensão da base da percepção sensorial e regulação do processamento neural que é importante na reabilitação e gestão da doença para seres humanos com anormalidades unidade motora (Patel et al, 2009;. Rabin et al, 2009;. Marino et al ., 2010). Os vários tipos de entrada e padrões de disparo para monitorar movimentos articulares na acessíveis preparações invertebrados podem ser usados ​​em robótica / prótese (Macmillan e Patullo, 2001). Há ainda muitas perguntas à espera de respostas nesta preparação que pode ser benéfica em um número de maneiras.

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muscle receptor organs in the crayfish abdomen: a student laboratory exercise in proprioception

O principal objetivo desse experimento é demonstrar primário informações neurônios sensoriais transporte de movimentos articulares e posições como as informações proprioceptivas para um animal. Um objetivo adicional deste experimento é o de aprender a anatomia da preparação por dissecção coloração e visualização de neurônios e estruturas sensoriais sob um microscópio de dissecação. Isto é realizado usando o equipamento neurofisiológico básico para registrar a atividade elétrica de um órgão receptor comum e técnicas de coloração. O músculo receptor de sistema de órgãos (MRO) da lagosta é análogo ao fuso muscular intrafusais em mamíferos, o que ajuda na servindo como modelo comparativo que é mais facilmente acessíveis para as gravações eletrofisiológicas. Além disso, estes são identificáveis ​​neurônios sensoriais entre as preparações. A preparação é viável em uma solução salina mínimo de horas que é favorável para os exercícios de laboratório dos alunos. A MRO é também suscetível a neuromodulação que incentiva questões intrigantes nos locais de ação moduladora e integração de sinais dinâmicos de movimentos e posição estática, juntamente com um ganho que pode ser alterado no sistema.

Watch this Scientific Journal Video about Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception at JoVE.com

Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception

O objetivo principal desta experiência é entender como os neurônios sensoriais primários transmitir informações de movimentos comuns e de posições como as informações proprioceptivas para um animal. Um objetivo adicional deste relatório é apresentar a anatomia da preparação por dissecção e visualização de neurônios em um microscópio de dissecação.

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Imagiologia pHluorin-tagged Inserção receptor para a membrana plasmática em primários de rato cultivadas Neurons

A better understanding of the mechanisms governing receptor trafficking between the plasma membrane (PM) and intracellular compartments requires an ...

Proprioception and Tension Receptors in Crab Limbs: Student Laboratory Exercises

Propriocepção e tensão Receptores em Caranguejo Limbs: Exercícios Laboratório Student

The primary purpose of these procedures is to demonstrate for teaching and research purposes how to record the activity of living primary sensory neurons ...

Measuring Oral Fatty Acid Thresholds, Fat Perception, Fatty Food Liking, and Papillae Density in Humans

Medindo Oral Ácido Limites Fatty, Fat Percepção, gordos alimentares Gostar, e Papilas Densidade em seres humanos

Emerging evidence from a number of laboratories indicates that humans have the ability to identify fatty acids in the oral cavity, presumably via fatty ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Monte todo imunomarcação de Neurônios Receptores Olfatórios no<em&gt; Drosophila</em&gt; Antena

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Um<em&gt; In Vitro</emPreparação Muscle-nervo&gt; Adulto mouse para estudar as propriedades de queima de músculos aferentes

Muscle sensory neurons innervating muscle spindles and Golgi tendon organs encode length and force changes essential to proprioception. Additional ...