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09:58 min
February 2nd, 2011
DOI :
10.3791/2348-v
Chapters
0:05
Title
1:27
Preparation of Reagents
2:09
Tissue Collection and Homogenization
2:56
Gradient Set Up
5:56
Antibody Staining
8:08
Representative Analysis by Flow Cytometry
9:29
Conclusion
Summary
The current article describes a rapid protocol to efficiently isolate mononuclear cells from brain and spinal cord tissues that can be effectively utilized for flow cytometric analyses.
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