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17:14 min
January 26th, 2011
DOI :
10.3791/2352-v
Chapters
1:34
Introduction
2:10
Generation of GFP-tagged Sla1 and Sla1 Clatherin-binding Mutant Yeast Strains
5:09
Fluorescence Image Analysis
3:22
Fluorescence Microscopy
6:41
Preparation of Yeast Cytosolic Extract
11:53
Preparation of Total Cell Extract
9:54
GST-fusion Protein Affinity Assay
13:36
Co-immunoprecipitation Assay
15:16
Representative Results
16:39
Conclusion
Summary
网格蛋白介导的内吞作用取决于适配器的蛋白质,协调货物选择和网格蛋白外衣大会。在这里,我们描述的程序来研究网格蛋白适配器的物理互动和活细胞成像的方法,作为一种模式使用酵母吞适配器蛋白Sla1p。
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