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32.6K Views
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15:40 min
January 24th, 2011
DOI :
10.3791/2373-v
Chapters
0:00
Title
2:23
Construction of Imaging Chambers
3:45
Cortical Glial Feeder Layer Preparation for Long-term Cultures
7:39
Cortical and/or Hippocampal Dissection and Electroporation
12:10
Representative Results
14:37
Conclusion
Summary
该协议概述解剖所需的步骤,通过电穿孔和文化小鼠海马和皮层神经元的转染。短期可用于文化研究轴突的生长和指导,而长期的文化可以为研究突触和树突棘分析。
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