Name: ex vivo culture of patient tissue &amp; examination of gene delivery
Uploaded: 2010-12-20T18:00:00
Description: Watch this Scientific Journal Video about Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery at JoVE.com

Detta arbete har finansierats genom Cork South Infirmary Victoria Universitetssjukhuset bröstcancer fond, den irländska Cancer Society (CRI07TAN) och Cork Cancer Research Centre. Replikering inkompetenta rekombinant Adenovirus 5 partiklar under transkriptionella kontroll av CMV promotor var en slags gåva från professor Andrew Baker, University of Glasgow.

Beredning av medier och reagenser <ol><li> Antibiotika Lösning Fosfatbuffrad saltlösning (PBS) med 200 U / ml penicillin, 200 mikrogram / ml streptomycin, 5 mikrogram / ml fungizone </li><li> Insamling och behandling medelstora Dulbecco ändrade eagle medium (DMEM) med 200 U / ml penicillin, 200 mikrogram / ml streptomycin, 5 mikrogram / ml fungizone </li><li> Odlingsmedium Insamling medium som innehåller 10% värmeinaktiveras fetalt bovinserum...

<ol>
	<li>van de Bovenkamp, M., Groothuis, G. M., Draaisma, A. L., Merema, M. T., Bezuijen, J. I., van Gils, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Precision-cut+liver+slices+as+a+new+model+to+study+toxicity-induced+hepatic+stellate+cell+activation+in+a+physiologic+milieu.">Precision-cut liver slices as a new model to study toxicity-induced hepatic stellate cell activation in a physiologic milieu.</a> Toxicol Sci. 85, 632-638 (2005).</li><li>Speirs, V., Green, A. R., Walton, D. S., Kerin, M. J., Fox, J. N., Carleton, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short-term+primary+culture+of+epithelial+cells+derived+from+human+breast+tumours.">Short-term primary culture of epithelial cells derived from human breast tumours.</a> Br J Cancer. 78, 1421-1429 (1998).</li><li>Kirby, T. O., Rivera, A., Rein, D., Wang, M., Ulasov, I., Breidenbach, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+ex+vivo+model+system+for+evaluation+of+conditionally+replicative+adenoviruses+therapeutic+efficacy+and+toxicity.">A novel ex vivo model system for evaluation of conditionally replicative adenoviruses therapeutic efficacy and toxicity.</a> Clin Cancer Res. 10, 8697-8703 (2004).</li><li>Josserand, V., Texier-Nogues, I., Huber, P., Favrot, M. C., Coll, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-invasive+in+vivo+optical+imaging+of+the+lacZ+and+luc+gene+expression+in+mice.">Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice.</a> Gene Ther. 14, 1587-1593 (2007).</li></ol>

Vi beskriver en ex vivo patienten metod vävnadsodling och självlysande detektionssystem för bedömning av genen leverans inom icke fasta mänsklig vävnad. Metoden erbjuder ett enkelt och reproducerbart sätt skivor odling vävnad. Den har stor potential eftersom den tillåter studiet av genen leverans till intakt mänsklig vävnad, analys av genen leverans till en mängd mänsklig vävnad, inklusive maligna vävnad och kan ge viktig information om effekterna av den höga grad av variabilitet mellan enskilda...

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<td>DMEM</td>
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<td>D8537</td>
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<td>Firefly Luciferin</td>
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<td>Biosynth, Switzerland</td>
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<td>Dermabond</td>
<td>&nbsp;</td>
<td>Johnson &amp; Johnson</td>
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ex vivo culture of patient tissue &amp; examination of gene delivery

Denna video beskriver användningen av patientens vävnad som en ex vivo-modell för studier av genen leverans. Färska patientens vävnad som erhålls vid tidpunkten för ingreppet är skivad och upprätthålls i kulturen. Den ex vivo modellsystem gör det möjligt för den fysiska leveransen av gener i intakta patientens vävnad och genuttryck analyseras av mareld avbildning med IVIS detektionssystem. Den självlysande system för upptäckt visar snabb och exakt kvantifiering av genuttryck inom enskilda skivor utan att behöva vävnaden offer. Denna skiva vävnadskultur system kan användas i en mängd olika vävnadstyper, inklusive normala och maligna vävnad och ger oss möjlighet att studera effekterna av heterogena karaktär intakt vävnad och den höga graden av variation mellan enskilda patienter. Denna modell system skulle kunna användas i vissa situationer som ett alternativ till djurmodeller och som en kompletterande prekliniska läge före inträde klinisk prövning.

Watch this Scientific Journal Video about Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery at JoVE.com

Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery

Denna artikel beskriver kulturen i patientens vävnad skivor för studier gen leverans och efterföljande analys av genuttryck med hjälp IVIS bioluminescens avbildning.

Ex vivo kultur Patient Tissue &amp; Undersökning av Gene Delivery

This video describes the use of patient tissue as an ex vivo model for the study of gene delivery.  Fresh patient tissue obtained at the time of surgery ...

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Ex Vivo Culture of Patient Tissue &amp; Examination of Gene Delivery

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