Name: facilitating the analysis of immunological data with visual analytic techniques
Uploaded: 2011-01-02T17:00:00
Description: Watch this Scientific Journal Video about Facilitating the Analysis of Immunological Data with Visual Analytic Techniques at JoVE.com

我们想感谢温哥华的可视化分析（爆）研究所的成员，为项目提供意见和建议。特别是，我们要感谢约翰布赖恩费舍尔，莳萝，和大卫Darvill。我们还要感谢他们的支持和有益的讨论Kollmann实验室成员。这项工作是支持的，由国家过敏和传染病研究所，国立卫生格兰特N01 AI50023研究所的一部分;过敏原罗富国教育资助07 - A1A和07 - B2B的;和迈克尔史密斯健康研究基金会。 TRK是从宝来惠康基金广告事业在生物医学科学奖由加拿大卫生研究培训资助加拿大儿童健康临床医师科学家计划研究所与患病儿童基金，儿童与家庭研究所合作，在部分支持（不列颠哥伦比亚省），妇女和儿童健康研究所（艾伯塔），省和马尼托巴省儿童健康研究所。

1。探索为基础的分析，对抽穗<ol><li>你有一个数据集，你想探索在数据列之间的各种关系。 <ol><li> （图1）点击“连接到数据”导入数据集。选择数据源类型，并按照说明导入您的数据集。 </li><li> （图1）确保尺寸货架包含断然措施货架包含的列，而从您的数据集的数值列。有时一个主题标识符，如维列可能被列为衡量，如果它是numerated。右键单击它，改变它的尺寸，并选择“转换尺寸”。在?...

<ol>
	<li>Thomas, J., Cook, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Illuminating+the+Path:+The+Research+and+Development+Agenda+for+Visual+Analytics.">Illuminating the Path: The Research and Development Agenda for Visual Analytics.</a> , (2005).</li><li>Steed, C., Swan, J., Jankun-Kelly, T., Fitzpatrick, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Guided+Analysis+of+Hurricane+Trends+Using+Statistical+Processes+Integrated+with+Interactive+Parallel+Coordinates.">Guided Analysis of Hurricane Trends Using Statistical Processes Integrated with Interactive Parallel Coordinates.</a> IEEE Symposium on Visual Analytics Science and Technology. , 19-26 (2009).</li><li>Saraiya, P., North, C., Lam, V., Duca, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Insight-Based+Longitudinal+Study+of+Visual+Analytics.">An Insight-Based Longitudinal Study of Visual Analytics.</a> IEEE Transactions on Visualization and Computer Graphics. 12, 1511-1522 (2006).</li><li>Kollmann, T. R., Crabtree, J., Rein-Weston, A., Blimkie, D., Thommai, F., Wang, X. Y., Lavoie, P. M., Furlong, J., Fortuno, E. S., Hajjar, A. M., Hawkins, N. R., Self, S. G., Wilson, C. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neonatal+innate+TLR-mediated+responses+are+distinct+from+those+of+adults.">Neonatal innate TLR-mediated responses are distinct from those of adults.</a> J. Immunol. 183, 7150-7160 (2009).</li></ol>

现代生物医学研究的高通量技术的问世，LED需要一个更有效的方法，分析研究数据的爆炸。可视化分析（VA）是通过交互式可视化界面（1​​）便利的分析推理的科学。在弗吉尼亚州的做法，恢复在分析人类的分析师手中的权力，违背传统的方法来检测电脑图案。可视化分析已在各个领域，如国防科研（1）和飓风的趋势（2），用于研究。到目前为止，有VA在生物学上的应用（3）只有几个例子。?...

facilitating the analysis of immunological data with visual analytic techniques

可视化分析（VA）已成为一种新的方式来分析大型数据集通过交互式可视化显示。我们证明了在弗吉尼亚州的生物数据集的分析方法的实用性和灵活性。在免疫学的这些数据集的例子包括流式细胞仪，Luminex公司的数据，和基因分型（如单核苷酸多态性）的数据。相反，传统的信息可视化方法，VA恢复在手中的分析师的分析权力，让分析师进行实时数据探索的过程。我们选择了在弗吉尼亚州的软件称为抽穗后评估了几个弗吉尼亚州的工具。在视频演示，使用的方法称为配对分析，两种类型的分析任务分析和数据集之间进行了论证。配对分析，在弗吉尼亚州的定义，是一个分析的方法，其中一个VA工具专家一个领域的专家在分析端侧。该领域的专家是谁理解数据的意义，并要求所收集的数据可能解决的问题之一。然后创建工具专家，以帮助寻找可能回答这些问题的数据模式的可视化。之间的假设生成和数据的快速可视化显示滞后时间短是一个VA方式的主要优势。

Watch this Scientific Journal Video about Facilitating the Analysis of Immunological Data with Visual Analytic Techniques at JoVE.com

Facilitating the Analysis of Immunological Data with Visual Analytic Techniques

可视化分析（VA）是一种新的交互方式分析数据的方法。在这段视频中，我们讨论的高通量生物实验所带来的数据超载的问题，并提出为解决这样的问题的弗吉尼亚州。视频演示和分析使用的是弗吉尼亚州的工具，称为抽穗的免疫学数据集之间的。

促进免疫学数据分析与视觉分析技术

Visual analytics (VA) has emerged as a new way to analyze large dataset through interactive visual display. We demonstrated the utility and the ...

Research

JoVE Journal

Immunology and Infection

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

促进药物发现：斑马鱼在一个自动化的高含量检测炎症

Zebrafish larvae are particularly amenable to whole animal small molecule screens1,2 due to their small size and relative ease of manipulation and ...

科研

免疫与感染

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

免疫突触由双色时间选通受激发射损耗（STED）纳米显微可视化

Natural killer cells form tightly regulated, finely tuned immunological synapses (IS) in order to lyse virally infected or tumorigenic cells. Dynamic ...

An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

成像免疫突触动力学内皮平面细胞模型

Adaptive immunity is regulated by dynamic interactions between T cells and antigen presenting cells (&#39;APCs&#39;) referred to as &#39;immunological ...

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

使用的淋巴细胞外渗分析<em&gt;体外</em&gt;人类血脑屏障模型

Lymphocyte extravasation into the central nervous system (CNS) is critical for immune surveillance. Disease-related alterations of lymphocyte ...

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

超 cyt: 一种用于简化高维细胞学数据分析的图形用户界面

With the advent of flow cytometers capable of measuring an increasing number of parameters, scientists continue to develop larger panels to phenotypically ...

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

在研究隐球菌-阿米巴相互作用中,显微技术和荧光读数的补充应用

To simulate Cryptococcus infection, amoeba, which is the natural predator of cryptococcal cells in the environment, can be used as a model for ...

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

评估细菌病原体免疫反应的声吞噬杀灭试验

A key aspect of the immune response to bacterial colonization of the host is phagocytosis. An opsonophagocytic killing assay (OPKA) is an experimental ...

Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes

在小鼠小腿上应用一致的按摩样扰动，并监测由此产生的肌肉压力变化

Massage is generally recognized to be beneficial for relieving pain and inflammation. Although previous studies have reported anti-inflammatory effects of ...

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

原子力显微镜罐体功能化,具有单T细胞或单粒子免疫单细胞力光谱

Atomic force microscopy based single cell force spectroscopy (AFM-SCFS) is a powerful tool for studying biophysical properties of living cells. This ...

Imaging the Human Immunological Synapse

成像人类免疫学突触

The purpose of the method is to generate an immunological synapse (IS), an example of cell-to-cell conjugation formed by an antigen-presenting cell (APC) ...