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14.7K Views
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09:14 min
March 17th, 2011
DOI :
10.3791/2409-v
Chapters
0:05
Title
1:49
Preparation of Coverslips or "Video" Dishes
3:40
Preparation of Neuronal Cultures and Media
4:53
Permeabilization of Neuronal Cultures and Incorporation of Rhodamine-actin onto F-actin Barbed Ends
6:13
Variations to this Procedure
7:35
Representative Actin Labeling Results
8:47
Conclusion
Summary
描述的方法来可视化和量化,F -肌动蛋白在神经生长锥刺完。盖玻片上培养神经细胞后,细胞透用含皂甙的解决方案。然后,潜伏期短皂苷含有若丹明-肌动蛋白的缓冲区纳入到免费的肌动蛋白刺完肌动蛋白的荧光。
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