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DOI :
10.3791/2409-v
March 17th, 2011
Chapters
0:05
Title
1:49
Preparation of Coverslips or "Video" Dishes
3:40
Preparation of Neuronal Cultures and Media
4:53
Permeabilization of Neuronal Cultures and Incorporation of Rhodamine-actin onto F-actin Barbed Ends
6:13
Variations to this Procedure
7:35
Representative Actin Labeling Results
8:47
Conclusion
神経成長円錐におけるF -アクチン有刺鉄線両端を視覚化し、定量化する方法が記載されている。ガラス製カバースリップ上で培養ニューロン後、細胞をサポニン含有溶液に透過処理されています。その後、ローダミン-アクチンを含むサポニンバッファと短いインキュベーションは無料アクチン有刺鉄線端上に蛍光アクチンを内蔵しています。
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