Name: methods for patch clamp capacitance recordings from the calyx
Uploaded: 2007-07-29T00:00:00
Description: Watch this Scientific Journal Video about Methods for Patch Clamp Capacitance Recordings from the Calyx at JoVE.com

膜片钳安装： 光学：光学找到一个良好的细胞，并确立它的地位是非常重要的。 尝试获取细胞的清晰的图像：锋利的边缘，软前瞻性细胞。 扫描整个领域寻找良好MNTB附有萼主要细胞。旋转盘，从不同的角度去寻找盏。首先，它是确定实践气球形的终端。 接片表面的细胞，或在第二届细胞层?...

methods for patch clamp capacitance recordings from the calyx

我们展示举行，哺乳动物的中枢神经系统的神经终端的花萼突触前膜片钳记录的基本技巧。从突触前终端电器录音允许的动作电位，钙通道电流，囊泡融合（胞吐）和随后的膜摄取（内吞作用）的测量。含有神经递质囊泡的融合导致囊膜被添加到细胞膜的花萼。在细胞膜量的增加是增加电容测量。随后的电容减少，表明细胞内吞，膜吸收或去除花萼膜的过程。内吞作用，是需要保持的花萼结构和形式将与未来的胞吐事件充满神经递质的囊泡，它也是必要的。萼举行的电容录音，可以直接和快速测量水泡在哺乳动物中枢神经系统的神经末端的释放和随后的内吞作用。此外，相应的突触后活动可同时测量通过使用配对录音。因此，一个完整的画面，在中枢神经系统的突触前和突触后的电活动是可以实现的，使用该制剂的。这里介绍的方法，片制备，鉴定举行，基本的膜片钳技术盏形态特征，和电容录音来衡量胞外分泌和内吞作用的例子。

Watch this Scientific Journal Video about Methods for Patch Clamp Capacitance Recordings from the Calyx at JoVE.com

Methods for Patch Clamp Capacitance Recordings from the Calyx

膜片钳从花萼电容录音方法

We demonstrate the basic techniques for presynaptic patch clamp recording at the calyx of Held, a mammalian central nervous system nerve terminal. ...

Research

JoVE Journal

Biology

Whole Cell Recordings from Brain of Adult Drosophila

从整个成年果蝇大脑细胞记录

In this video, we demonstrate the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons. We begin by ...

科研

生物学

Patch Clamp Recording of Ion Channels Expressed in  Xenopus Oocytes

补丁钳爪蟾卵母细胞中表达的离子通道

Since its development by Sakmann and Neher 1, 2, the patch clamp has become established as an extremely useful technique for electrophysiological ...

Pressure-polishing Pipettes for Improved Patch-clamp Recording

改进的膜片钳记录的压力抛光移液器

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording.  We first developed this method for fabricating pipettes suitable for ...

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

电生理方法记录从果蝇幼虫NMJ突触潜力

In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. ...

Single-cell Suction Recordings from Mouse Cone Photoreceptors

单从小鼠锥感光细胞吸录音

Rod and cone photoreceptors in the retina are responsible for light detection. In darkness, cyclic nucleotide-gated (CNG) channels in the outer segment ...

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

膜片钳和灌注技术研究离子通道的表达爪蟾卵母细胞

The protocol presented here is designed to study the activation of the large conductance, voltage- and Ca2+-activated K+ (BK) channels. The protocol may ...

Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography

探讨动脉平滑肌的KV7钾离子通道功能使用膜片钳电生理和压力Myography

Contraction  or relaxation of smooth muscle cells within the walls of resistance arteries  determines the artery diameter and thereby controls flow of ...

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

巨脂质体制剂成像和膜片钳电

The reconstitution of ion channels into chemically defined lipid membranes for electrophysiological recording has been a powerful technique to identify ...

One-channel Cell-attached Patch-clamp Recording

单通道细胞贴附膜片钳记录

Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate ...

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

跨膜蛋白的重组，电压门控离子通道，KvAP，进入巨人单层囊的显微镜和膜片钳研究

Giant Unilamellar Vesicles (GUVs) are a popular biomimetic system for studying membrane associated phenomena. However, commonly used protocols to grow ...