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Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

DOI :

10.3791/2444-v

12:09 min

May 2nd, 2011

May 2nd, 2011

41,970 Views

1Lindsley F. Kimball Research Institute, New York Blood Center

This protocol describes a general procedure for studying recombinant receptor-binding domain (RBD)-based subunit vaccines against SARS. It includes methods for transfection and expression of RBD protein in 293T cells, immunization of mice with RBD and detection of neutralization activity of mouse sera using an established SARS pseudovirus neutralization assay.

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Recombinant RBD based SARS Vaccines

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