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28.4K Views
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09:45 min
March 30th, 2011
DOI :
10.3791/2452-v
Chapters
0:05
Title
1:15
Muscle Isolation/Homogenization and Mitochondrial Isolation
4:13
Electrode Calibration
6:17
Mitochondrial Respiration
8:15
Representative Mitochondrial Respiration Results
9:15
Conlcusion
Summary
这个协议描述一个过程来研究从骨骼肌中分离出的线粒体呼吸。这种方法改编自Scorrano等。(2007年)。线粒体的分离过程中需要约2小时。线粒体呼吸在大约1个小时就可以完成。
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