Name: the use of drip flow and rotating disk reactors for staphylococcus aureus biofilm analysis
Uploaded: 2010-12-27T17:00:00
Description: Watch this Scientific Journal Video about The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis at JoVE.com

NIAID的赠款K22AI081748。

1。滴流生物膜反应器<ol><li>滴流生物膜反应器（Biosurface技术或定制设计的版本，通常可以由大学机械厂，看到图1）是组装和​​灭菌。大会涉及商会和保护室盖张贴券。随着生物膜的介质（胰蛋白酶大豆肉汤2克/ L和葡萄糖2克/升），和营养管进水室，高压灭菌消毒。 </li><li>接种的滴流式反应器是预制反应器放置在一个平面上，夹紧污水管线，灌装用10 mL的胰蛋白酶大豆肉汤每个分庭，加入1...

<ol>
	<li>Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R., Lappin-Scott, H. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbial+Biofilms.">Microbial Biofilms.</a> Annu. Rev. Microbiol. 49, 711-745 (1995).</li><li>Costerton, J. W., Cheng, K. J., Gessey, G. G., Ladd, T. I., Nickel, J. C., Dasgupta, M., Marrie, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bacterial+biofilms+in+nature+and+disease.">Bacterial biofilms in nature and disease.</a> Ann. Rev. Microbiol. 41, 435-464 (1987).</li><li>O'Toole, G. A., Kolter, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Initiation+of+biofilm+formation+in+Pseudomonas+fluorescens+WCS365+proceeds+via+multiple,+convergent+signaling+pathways:+a+genetic+analysis.">Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signaling pathways: a genetic analysis.</a> Mol. Micro. 28, 449-461 (2002).</li><li>Boles, B. R., Horswill, A. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Agr-mediated+dispersal+of+Staphylococcus+aureus+biofilms.">Agr-mediated dispersal of Staphylococcus aureus biofilms.</a> PLoS Pathog. 4, e1000052-e1000052 (2008).</li><li>Goeres, D. M., Haamilton, M. A., Beck, N. A., Buckingham-Meyer, K., Hilyard, J., Loetterle, L. A., Walker, D. K., Stewart, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+method+for+growing+a+biofilm+under+low+shear+at+the+air-liquid+interface+using+the+drip+flow+biofilm+reactor.">A method for growing a biofilm under low shear at the air-liquid interface using the drip flow biofilm reactor.</a> Nature Protocols. 4, 783-788 (2009).</li><li>Fu, W., Forster, T., Mayer, O., Curtin, J. J., Lehman, S. M., Donlan, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bacteriophage+cocktail+for+the+prevention+of+biofilm+formation+by+Pseudomonas+aeruginosa+on+catheters+in+an+in+vitro+model+system.">Bacteriophage cocktail for the prevention of biofilm formation by Pseudomonas aeruginosa on catheters in an in vitro model system.</a> Antimicrob Agents Chemother. 54, 397-404 (2010).</li><li>Xu, K. D., McFeters, G. A., Stewart, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biofilm+resistance+to+antimicrobial+agents.">Biofilm resistance to antimicrobial agents.</a> Microbiology. 146, 547-549 (2000).</li><li>Xu, K. D., Stewart, P. S., Xia, F., Huang, C. T., McFeters, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spatial+physiological+heterogeneity+in+Pseudomonas+aeruginosa+biofilm+is+determined+by+oxygen+availability.">Spatial physiological heterogeneity in Pseudomonas aeruginosa biofilm is determined by oxygen availability.</a> Appl. Environ. Microbiol. 64, 4035-4039 (1998).</li><li>Boles, B. R., Thoendel, M., Singh, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-generated+diversity+produces+"insurance+effects"+in+biofilm+communities.">Self-generated diversity produces "insurance effects" in biofilm communities.</a> Proc. Natl. Acad. Sci. USA. 101, 16630-16635 (2004).</li><li>Pitts, B., Willse, A., McFeters, G. A., Hamilton, M. A., Zelver, N., Stewart, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+repeatable+laboratory+method+for+testing+the+efficacy+of+biocides+against+toilet+bowl+biofilms.">A repeatable laboratory method for testing the efficacy of biocides against toilet bowl biofilms.</a> J. Appl. Microbiol. 91, 117-11 (2001).</li><li>Boles, B. R., Thoendel, M., Singh, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rhamnolipids+mediate+detachment+of+Pseudomonas+aeruginosa+from+biofilms.">Rhamnolipids mediate detachment of Pseudomonas aeruginosa from biofilms.</a> Mol. Microbiol. 57, 1210-1223 (2005).</li><li>Hentzer, M., Teitzel, G. M., Balzer, G. J., Heydorn, A., Molin, S., Givskov, M., Parsek, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alginate+overproduction+affects+Pseudomonas+aeruginosa+biofilm+structure+and+function.">Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function.</a> J. Bacteriol. 183, 5395-5401 (2001).</li><li>Lin, H. Y., Chen, C. T., Huang, C. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+merocyanine+540+for+photodynamic+inactivation+of+Staphylococcus+aureus+planktonic+and+biofilm+cells.">Use of merocyanine 540 for photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells.</a> Appl. Environ. Microbiol. 70, 6453-6458 (2004).</li></ol>

在不同的反应堆生长的生物膜，往往会产生不同的特点和每个反应器具有不同的应用。在这项工作中，我们描述了两个生物膜反应器的使用：滴流生物膜反应器和一个旋转的磁盘反应堆。滴流电抗器是有用和越来越低气 - 液界面剪切生物膜，适应各种条件。我们发现大量的生物膜生物量是可取的研究极为方便。这种设置可以很容易地适应对涉及的微传感器的监测和测试潜在的antibiofilm表面研究。 <...

Erratum: The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

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<td>Drip Flow Reactors</td>
<td>&nbsp;</td>
<td>BioSurface Technologies Corporation</td>
<td>DFR 110</td>
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<td>Rotating Disk Reactors</td>
<td>&nbsp;</td>
<td>BioSurface Technologies Corporation</td>
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the use of drip flow and rotating disk reactors for staphylococcus aureus biofilm analysis

被认为是自然界中最微生物在生物膜表面相关的社区存在。细菌生物膜 1矩阵内的包裹和连接表面生物膜的形成和发展，通常是在实验室使用批处理系统，如微孔板或流量的研究系统，如流细胞。这些方法，筛选突变体和化学库（微孔板）可视化（流动池）4 3或生长生物膜。在这里，我们提出两种其他类型的流量系统生物膜生长在金黄色葡萄球菌的详细协议：滴流生物膜反应器和生物膜反应器的旋转盘。 滴流生物膜反应器是专为低剪切条件下生长的生物膜的研究，5滴流式反应器由四个平行测试通道，每持有一个标准玻璃显微镜玻片大小的优惠券，或导管或限制的长度。滴流式反应器是理想的微传感器监测，一般生物膜研究，生物膜cryosectioning样品，高生物量生产，医用材料的评价，并留置医疗器械检测 6,7,8,9 。 旋转圆盘式反应器由聚四氟乙烯包含可移动优惠券的凹槽的磁盘。10可拆卸的优惠券可以由任何可加工材料。旋转盘的底部有一个条形磁铁，让创建跨磁盘旋转液体表面剪切表面冲洗券。 1000毫升的玻璃侧臂的反应堆压力容器是摆在整个磁盘，其中包含18券。液体增长媒体是通过船只的分发，而磁盘是由磁力搅拌器旋转。优惠券是从反应堆压力容器，然后刮下收集生物膜样本作进一步研究或显微成像。旋转圆盘反应器是专为实验室生物杀灭剂的功效，去除生物膜和防污材料的性能评价。9,11,12,13

Watch this Scientific Journal Video about The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis at JoVE.com

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

详细介绍了利用开放系统流生物膜与滴流反应器和旋转磁盘反应堆的协议。

滴流和旋转盘反应器使用金黄色葡萄球菌生物膜分析

Most microbes in nature are thought to exist as surface-associated communities in biofilms.1  Bacterial biofilms are encased within a matrix and attached ...

Research

JoVE Journal

Immunology and Infection

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

铜绿假单胞菌和酿酒酵母流细胞生物膜

Many microbial cells have the ability to form sessile microbial communities defined as biofilms that have altered physiological and pathological ...

科研

免疫与感染

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA)

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

皮下感染耐甲氧西林金黄色葡萄球菌（MRSA）的

MRSA is a worldwide threat to public health, and MRSA skin and soft-tissue infections now account for more than half of all soft-tissue infections in the ...

Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus (MRSA) in Rat

Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus MRSA in Rat

耐甲氧西林实验性心内膜炎模型<em&gt;金黄色葡萄球菌</em&gt;（MRSA）的大鼠

Endovascular infections, including endocarditis, are life-threatening infectious syndromes1-3. Staphylococcus aureus is the most common world-wide cause ...

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

金黄色葡萄球菌生长使用人血红蛋白作为铁源

S. aureus is a pathogenic bacterium that requires iron to  carry out vital metabolic functions and cause disease. The most abundant  reservoir of iron ...

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

??PCR?????????????????????MEC??????V<em&gt;???????</em

Staphylococcal Cassette Chromosome mec (SCCmec) typing  is a very important molecular tool for understanding the epidemiology and  clonal strain ...

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

相互作用的研究<em&gt;金黄色葡萄球菌</em与中性粒细胞通过流式细胞仪和时间推移显微镜

We present methods to study the effect of phenol soluble modulins (PSMs)  and other toxins produced and secreted by Staphylococcus  aureus on neutrophils. ...

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay

针对相关生物膜<em&gt;金黄色葡萄球菌</em&gt;使用基于刃天青药物敏感性测定

Most pathogenic bacteria are able to form biofilms during infection, but due to the difficulty of manipulating and assessing biofilms, the vast majority ...

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

基因分型<em&gt;金黄色葡萄球菌</em&gt;由核糖体垫片的PCR（RS-PCR）

The ribosomal spacer PCR (RS-PCR) is a highly resolving and robust genotyping method for S. aureus that allows a high throughput at moderate costs and is, ...

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

方法基因水平转移的研究<em&gt;金黄色葡萄球菌</em

One important feature of the major opportunistic human pathogen Staphylococcus aureus is its extraordinary ability to rapidly acquire resistance to ...

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

全血流式细胞术分析人单核细胞亚群的表征

Monocytes are key contributors in various inflammatory disorders and alterations to these cells, including their subset proportions and functions, can ...