Name: an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain
Uploaded: 2010-12-11T16:00:00
Description: Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

我们感谢丹McWhorter在视频叙述议定书。这项工作是由美国国立卫生研究院批准5R01NS062182，衰老研究从美国联邦补助金，并授予HTG机构资金支持。

一，程序下列技术应在无菌条件下进行，在层流罩，使用消毒工具。 器官片玻璃底部菜的制备<ol><li>菜肴必须在无菌环境下编写的，使用消毒工具。 </li><li>一个片介质150μL下降（见食谱）被放置在玻璃底部的一部分菜小心，以避免气泡在中期中心。 </li><li>随着一个一次性注射器与一个23计针，胶水多个点（橡皮泥 - 埃尔默的，猫＃E904）配备都放置在邻近?...

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在RMS的神经元的迁移，是产后神经发生在1嗅球的重要组成部分。通过RMS的迁移发生在大脑表面相切的平面。切向迁移的神经母细胞是从他们的祖源的位置，以及其神经元的最终产品1，2，3的发散命运的放射状迁移细胞的独特。切线方向迁移的细胞在产后RMS相对纯净的人口使这种解剖学上定义的区域研究切向迁移的机制的最佳平台。破译神经细胞迁移的细胞和分子机制的关键是理?...

an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain

产后大脑中的神经再生取决于三个生物事件的保养：祖细胞，成神经细胞迁移，以及分化和整合到现有的神经回路的新的神经元的增殖。在嗅球产后神经发生，这些事件被隔离在三个不同的域解剖学：扩散主要是发生在侧脑室室管膜下区（SEZ），移植的神经母细胞遍历喙迁徙流（RMS），和新的神经细胞分化和内的嗅球（OB）的整合。这三个领域作为理想的平台来研究细胞，分子和生理机制调节的生物事件明显。本文介绍了一个器官产后脑组织切片检测进行了优化，其中外条件紧密地模仿迁移神经母细胞在体内环境。我们表明，我们的检测提供了统一，导向，迅速运动神经母细胞内的RMS。此法将是非常适合的细胞自主和非自主神经细胞迁移的调节，利用交叉移植的方法，从不同的遗传背景的小鼠的研究。

Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

本协议描述了一个器官的切片检测，产后脑和延髓迁徙流时间推移高分辨率成像成神经细胞迁移的优化。

对于高分辨率延时成像神经细胞迁移的一个器官型产后脑切片检测

Neurogenesis in the postnatal brain depends on maintenance of three biological events: proliferation of progenitor cells, migration of neuroblasts, as ...

Research

JoVE Journal

Neuroscience

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

海马脑片的器官型检查神经元损伤的文化模式

Organotypic hippocampal slice culture is an in vitro method to examine mechanisms of neuronal injury in which the basic architecture and composition of ...

科研

神经科学

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

在发展中国家斑马鱼的前脑的神经祖细胞克隆相关的定时实时成像

Precise patterns of division, migration and differentiation of neural progenitor cells are crucial for proper brain development and function1,2. To ...

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

器官型培养的神经元雪崩多电极阵列记录

The cortex is spontaneously active, even in the absence of any particular input or motor output.  During development, this activity is important for the ...

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor

在犁鼻器官的切片准备工作表示一个基因编码的钙传感器成像神经元的反应

The vomeronasal organ (VNO) detects chemosensory signals that carry information about the social, sexual and reproductive status of the individuals within ...

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

高分辨率人类中脑功能磁共振成像方法

Functional MRI (fMRI) is a widely used tool for non-invasively measuring correlates of human brain activity. However, its use has mostly been focused upon ...

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

急性片的成年小鼠前脑的神经细胞迁移的延时成像

There is a substantial body of evidence indicating that new functional neurons are constitutively generated from an endogenous pool of neural stem cells ...

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

成神经细胞迁移的小鼠急性脑片在体内电穿孔产后和时间推移成像

The subventricular zone (SVZ) is one of the main neurogenic niches in the postnatal brain. Here, neural progenitors proliferate and give rise to ...

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

小脑颗粒神经元的遗传操作<em&gt;体外</em&gt;和<em&gt;在体内</em&gt;为研究神经元形态和迁移

Developmental events in the brain including neuronal morphogenesis and migration are highly orchestrated processes. In vitro and in vivo analyses allow ...

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

高分辨率<em&gt;在体内</em&gt;使用3T磁共振成像手动分割协议为人类海马子字段

The human hippocampus has been broadly studied in the context of memory and normal brain function and its role in different neuropsychiatric disorders has ...

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

延期共聚焦显像移植神经元在器官性切片培养胚胎小鼠脑使用<em&gt;在Utero</em&gt;电穿孔

In utero electroporation is a rapid and powerful approach to study the process of radial migration in the cerebral cortex of developing mouse embryos. It ...