Name: an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain
Uploaded: 2010-12-11T16:00:00
Description: Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

Nous tenons à remercier Dan McWhorter pour raconter le protocole dans la vidéo. Ce travail est soutenu par des subventions du NIH 5R01NS062182, une subvention de la Fédération américaine pour la recherche sur le vieillissement, et les fonds institutionnels attribué à HTG.

I. Procédures Les techniques suivantes doivent être effectuées dans des conditions stériles, dans une hotte à flux laminaire, en utilisant des outils stérilisés. Préparation de plats à fond de verre pour les tranches organotypiques <ol><li> Plats doit être préparé en milieu stérile et en utilisant des outils stérilisés. </li><li> Une baisse de 150μL de milieu tranche (voir recettes) est placé dans le centre de la partie en verre-fond du plat avec ...

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La migration neuronale dans le RMS est une composante essentielle de la neurogenèse postnatale dans les bulbes olfactifs 1. La migration à travers le RMS se produit dans un plan tangentiel à la surface du cerveau. Neuroblastes migrent tangentiellement sont distinctes de la migration des cellules radialement basés sur la localisation de leur source progénitrices, ainsi que le sort de leurs divergences produits finis neuronale 1, 2, 3. La population est relativement pure de cellules migrent tang...

an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain

Neurogenèse dans le cerveau postnatal dépend du maintien de trois événements biologiques: la prolifération des cellules progénitrices, la migration des neuroblastes, ainsi que la différenciation et l&#39;intégration de nouveaux neurones dans les circuits neuronaux existants. Pour neurogenèse postnatale dans les bulbes olfactifs, ces événements sont séparés dans les trois domaines anatomiquement distincts: la prolifération se produit essentiellement dans la zone sous épendymaire (ZES) des ventricules latéraux, la migration des neuroblastes parcourir à travers le flux migratoire rostral (RMS), et de nouveaux neurones se différencier et intégrer dans les bulbes olfactifs (OB). Les trois domaines servent de plateformes idéales pour étudier les mécanismes cellulaires, moléculaires et physiologiques qui régulent chacun des événements biologiques distinctement. Ce document décrit un essai organotypique optimisé pour les tissus du cerveau après la naissance, dans lequel les conditions extracellulaires imitent l&#39;environnement in vivo pour la migration des neuroblastes. Nous montrons que notre test fournit pour le mouvement uniforme, orienté et rapide des neuroblastes dans le RMS. Ce test sera très approprié pour l&#39;étude de la régulation des cellules autonomes et non autonomes de la migration neuronale en utilisant des approches inter-greffe de la souris sur les différents fonds génétiques.

Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Ce protocole décrit une analyse organotypique optimisé pour le cerveau postnatal et à haute résolution imagerie time-lapse de la migration des neuroblastes dans le flux rostrale migrateurs.

Un test organotypique à haute résolution imagerie time-lapse de la migration neuronale dans le cerveau postnatal

Neurogenesis in the postnatal brain depends on maintenance of three biological events: proliferation of progenitor cells, migration of neuroblasts, as ...

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Neuroscience

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

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Organotypic hippocampal slice culture is an in vitro method to examine mechanisms of neuronal injury in which the basic architecture and composition of ...

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Neurosciences

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Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

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L&#39;imagerie confocale temporelle des neurones migrateurs dans la culture de tranches organotypiques du cerveau embryonnaire de souris en utilisant<em&gt; Dans Utero</em&gt; Electroporation

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