Name: an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain
Uploaded: 2010-12-11T16:00:00
Description: Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

Wir danken Dan für McWhorter erzählen das Protokoll in dem Video. Diese Arbeit wird durch NIH Grant 5R01NS062182, einen Zuschuss von der American Federation for Aging Research, und institutionelle Fonds verliehen HTG unterstützt.

I. Verfahren Die folgenden Techniken sollten unter sterilen Bedingungen durchgeführt werden, in einer Sterilbank mit sterilisierten Instrumente. Vorbereitung der Glasboden Gerichte für organotypischen Schnitten <ol><li> Gerichte müssen in sterilen Umgebung zubereitet und mit sterilisierten Instrumente. </li><li> A 150 &amp;mgr; l Tropfen Scheibe Medium (siehe Rezepte) befindet sich im Zentrum der Glas-Unterteil der Schale mit Sorgfalt, um Luftblasen in das Medi...

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Neuronale Migration in der RMS ist ein wesentlicher Bestandteil der postnatalen Neurogenese im Riechkolben 1. Migration durch die RMS erfolgt in einer Ebene tangential zur Oberfläche des Gehirns. Tangential wandernden Neuroblasten unterscheiden sich von radial wandernden Zellen auf die Lage ihrer Vorläuferzellen Quelle sowie die divergente Schicksal ihrer endgültigen neuronalen Produkte 1, 2, 3. Die relativ reine Population von tangential wandernden Zellen in der postnatalen RMS macht diese anat...

an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain

Neurogenese im postnatalen Gehirn hängt Wartung von drei biologischen Ereignisse: die Proliferation von Vorläuferzellen, die Migration von Neuroblasten, sowie die Differenzierung und Integration neuer Neuronen in bestehende neuronale Schaltkreise. Für die postnatale Neurogenese im Riechkolben, werden diese Ereignisse innerhalb von drei anatomisch verschiedene Domains getrennt: Verbreitung weitgehend erfolgt in der subependymale Zone (SEZ) der Seitenventrikel, Migration Neuroblasten durch den rostralen wandernden Strom (RMS) durchlaufen, und neue Neuronen zu differenzieren und Integration innerhalb der Riechkolben (OB). Die drei Domänen dienen als ideale Plattformen, um die zellulären, molekularen und physiologischen Mechanismen, die jedem der biologischen Ereignisse deutlich regulieren zu studieren. Dieses Papier beschreibt eine organotypischen Slice-Assay für die postnatale Hirngewebe optimiert, in denen die extrazelluläre Bedingungen genau imitieren die in vivo-Umgebung für die Migration von Neuroblasten. Wir zeigen, dass unser Assay für eine einheitliche, orientiert und schnelle Bewegung der Neuroblasten innerhalb der RMS bietet. Dieser Assay wird als sehr geeignet für die Untersuchung von Zell-autonome und nicht autonome Regulation neuronaler Migration durch die Nutzung Cross-Transplantation Ansätze von Mäusen auf unterschiedlichen genetischen Hintergründen.

Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Dieses Protokoll beschreibt eine organotypischen Slice-Assay für die postnatale Gehirn und hochauflösenden Zeitraffer-Bildgebung von Neuroblasten Migration in der rostralen wandernde Stream optimiert.

Ein organotypischen Assay für High-Resolution Time-Lapse Imaging neuronaler Migration in der postnatalen Gehirn

Neurogenesis in the postnatal brain depends on maintenance of three biological events: proliferation of progenitor cells, migration of neuroblasts, as ...

Research

JoVE Journal

Neuroscience

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

Die Organotypische hippokampalen Slice-Kultur-Modell für die Prüfung neuronaler Schädigung

Organotypic hippocampal slice culture is an in vitro method to examine mechanisms of neuronal injury in which the basic architecture and composition of ...

Forschung

Neurowissenschaften

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

Zeitraffer-Live-Imaging von klonal verbundenen neuronalen Vorläuferzellen in der Entwicklung von Zebrafisch Vorderhirnneuronen

Precise patterns of division, migration and differentiation of neural progenitor cells are crucial for proper brain development and function1,2. To ...

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

Multi-Elektroden-Array Recordings neuronaler Lawinen in organotypischen Kulturen

The cortex is spontaneously active, even in the absence of any particular input or motor output.  During development, this activity is important for the ...

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor

Imaging neuronalen Antworten in Scheiben Zubereitungen von Vomeronasalorgan Ausdruck eines genetisch kodierte Kalzium-Sensor

The vomeronasal organ (VNO) detects chemosensory signals that carry information about the social, sexual and reproductive status of the individuals within ...

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

Hochauflösende Functional Magnetic Resonance Imaging Methods for Human Mittelhirn

Functional MRI (fMRI) is a widely used tool for non-invasively measuring correlates of human brain activity. However, its use has mostly been focused upon ...

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

Zeitraffer-Imaging von Neuroblast Migration in Acute Scheiben von der adulten Maus Vorderhirnneuronen

There is a substantial body of evidence indicating that new functional neurons are constitutively generated from an endogenous pool of neural stem cells ...

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In-vivo-Elektroporation und Postnatale Zeitraffer-Imaging Neuroblast von Migration in Maus Akute Hirnschnitten

The subventricular zone (SVZ) is one of the main neurogenic niches in the postnatal brain. Here, neural progenitors proliferate and give rise to ...

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetische Manipulation von Kleinhirnkörnerzellen<em&gt; In-vitro-</em&gt; Und<em&gt; In Vivo</em&gt;, Um neuronale Morphologie und Migration Studieren

Developmental events in the brain including neuronal morphogenesis and migration are highly orchestrated processes. In vitro and in vivo analyses allow ...

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Hohe Auflösung<em&gt; In-vivo-</em&gt; Manuelle Segmentierung Protokoll für Menschen Hippocampus Teilfelder Verwenden von 3T Kernspintomographie

The human hippocampus has been broadly studied in the context of memory and normal brain function and its role in different neuropsychiatric disorders has ...

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Zeitraffer-konfokale Bildgebung der Migration von Neuronen in der organotypischen Scheibe Kultur der embryonalen Maus Gehirn mit<em&gt; In Utero</em&gt; Elektroporation

In utero electroporation is a rapid and powerful approach to study the process of radial migration in the cerebral cortex of developing mouse embryos. It ...