Name: an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain
Uploaded: 2010-12-11T16:00:00
Description: Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

Vi tackar Dan McWhorter för berätta protokollet i videon. Detta arbete stöds av NIH Grant 5R01NS062182, ett bidrag från American Federation för Aging Research, och institutionella medel som beviljats ​​HTG.

I. Förfaranden Följande tekniker bör utföras under sterila förhållanden, i ett laminärt flöde huva, med steriliserade verktyg. Beredning av maträtter glas botten för organotypic skivor <ol><li> Rätter måste utarbetas i steril miljö och använder steriliserade verktyg. </li><li> En 150μL droppe skiva medium (se recept) är placerad i mitten av glaset-nedre delen av skålen med försiktighet för att undvika luftbubblor i mediet. </li><li> Med en engå...

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Neuronala migration i RMS är en viktig del av postnatal neurogenes i luktsinnet lökar 1. Migration genom RMS sker i ett plan som tangerar ytan av hjärnan. Tangentiellt migrera neuroblaster skiljer sig från radiellt migrera celler baserat på lokaliseringen av deras stamceller källa, liksom de skiljaktiga öde deras slutliga neuronala produkterna 1, 2, 3. Den relativt rena befolkning tangentiellt vandrar celler under den postnatala RMS gör detta anatomiskt definierbara regionen en optimal plat...

an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain

Neurogenes i den postnatala hjärnan beror på underhåll av tre biologiska händelser: spridning av progenitorceller, migration av neuroblaster samt differentiering och integration av nya nervceller i existerande neurala kretsar. För postnatal neurogenes i lukt lökar, är dessa händelser segregerade inom tre anatomiskt skilda områden: spridning till stor del sker i subependymala zon (SEZ) i den laterala ventriklarna, vandrar neuroblaster passera genom rostralt vandrande ström (RMS), och nya nervceller differentiera och integreras inom luktsinnet lökar (OB). De tre områden utgör idealiska plattformar för att studera de cellulära, molekylära och fysiologiska mekanismer som reglerar vart och ett av biologiska händelser tydligt. Detta dokument beskriver en organotypic slice analys optimerad för postnatal hjärnvävnad, där den extracellulära villkoren noga efterlikna in vivo-miljö för flyttande neuroblaster. Vi visar att vår analys ger enhetlig, orienterad, och snabb förflyttning av neuroblaster inom RMS. Denna analys kommer att vara mycket lämplig för studier av cellens autonoma och icke-självständiga reglering av neuronala migreringen genom att använda cross-transplantation metoder från möss på olika genetiska bakgrunder.

Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Detta protokoll beskriver en organotypic slice analys optimerad för den postnatala hjärnan och högupplösta tidsförlopp avbildning av neuroblast migrationen i rostralt flyttande strömmen.

En Organotypic Slice-analys för högupplöst Time-lapse avbildning av neuronala migration i Postnatal Brain

Neurogenesis in the postnatal brain depends on maintenance of three biological events: proliferation of progenitor cells, migration of neuroblasts, as ...

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Neuroscience

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

Den Organotypic hippocampus Slice Kultur Modell för prövningen Neuronal skada

Organotypic hippocampal slice culture is an in vitro method to examine mechanisms of neuronal injury in which the basic architecture and composition of ...

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

Time-lapse Live avbildning av kloner som är närbesläktade neurala stamceller i utvecklingsländerna Zebrafish framhjärnan

Precise patterns of division, migration and differentiation of neural progenitor cells are crucial for proper brain development and function1,2. To ...

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

Multi-elektrod Array Inspelningar av neuronala laviner i Organotypic kulturer

The cortex is spontaneously active, even in the absence of any particular input or motor output.  During development, this activity is important for the ...

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor

Imaging Neuronal svar i Slice Beredningar av vomeronasala Orgel uttrycker en genetiskt kodade Kalcium Sensor

The vomeronasal organ (VNO) detects chemosensory signals that carry information about the social, sexual and reproductive status of the individuals within ...

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

Högupplösta functional magnetic resonance imaging metoder för Human mitthjärnan

Functional MRI (fMRI) is a widely used tool for non-invasively measuring correlates of human brain activity. However, its use has mostly been focused upon ...

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

Time-lapse avbildning av Neuroblast Migration i akuta skivor av vuxen mus framhjärnan

There is a substantial body of evidence indicating that new functional neurons are constitutively generated from an endogenous pool of neural stem cells ...

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal elektroporation och Time-lapse avbildning av neuroblast migration i mus Akut hjärnan skivor

The subventricular zone (SVZ) is one of the main neurogenic niches in the postnatal brain. Here, neural progenitors proliferate and give rise to ...

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation av Cerebellar granulneuroner<em&gt; In Vitro</em&gt; Och<em&gt; In Vivo</em&gt; Att studera neuronal morfologi och migration

Developmental events in the brain including neuronal morphogenesis and migration are highly orchestrated processes. In vitro and in vivo analyses allow ...

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Hög upplösning<em&gt; In Vivo</em&gt; Manuell segmente Protocol for Human hippocampus delområden Använda 3T Magnetic Resonance Imaging

The human hippocampus has been broadly studied in the context of memory and normal brain function and its role in different neuropsychiatric disorders has ...

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Time-lapse-konfokalbildning av migrerande neuroner i organotypisk skivkultur av embryonalt mushinne<em&gt; I Utero</em&gt; Elektroporation

In utero electroporation is a rapid and powerful approach to study the process of radial migration in the cerebral cortex of developing mouse embryos. It ...