Name: an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain
Uploaded: 2010-12-11T16:00:00
Description: Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

Dan McWhorter video protokol anlatan için teşekkür ederiz. Bu çalışma NIH Hibe 5R01NS062182 Yaşlanma Araştırma Amerikan Federasyonu hibe ve HTG verilen kurumsal fonlar tarafından desteklenmektedir.

I. Prosedürleri Aşağıdaki teknikler, sterilize araçlarını kullanarak, bir laminer akış kaputu steril koşullar altında olmalıdır. Organotipik dilimler için cam alt yemeklerin hazırlanması <ol><li> Yemekleri steril bir ortamda hazırlanan ve araçlarını kullanarak sterilize edilmelidir. </li><li> 150μL dilim orta bir damla (yemek tarifleri) orta hava kabarcıklarını önlemek için dikkatli bir şekilde çanak cam alt kısmının merkezinde yer a...

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RMS Nöronal göç koku ampuller 1 postnatal nöron önemli bir bileşeni. Göç yoluyla RMS beynin yüzeyine teğet bir düzlem oluşur. Teğet göç neuroblasts radyal yanı sıra, son nöronal ürünler 1, 2, 3 ıraksak kaderi kendi progenitör kaynağının yerini dayalı hücreler göç farklıdır. Postnatal RMS teğet göç hücrelerinin oldukça saf bir nüfus bu anatomik olarak tanımlanabilir bölge teğet göç mekanizmaları çalışmak için optimum bir platform yapar. Nöronal göçü...

an organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain

Progenitör hücrelerin, neuroblasts göç yanı sıra, mevcut nöral devrelerin farklılaşma ve yeni nöronlar entegrasyonu yayılması: doğum sonrası beyindeki nöron üç biyolojik olaylar bakım bağlıdır. Koku ampuller doğum sonrası nöron için, bu olayların üç anatomik olarak farklı alanlar içinde ayrı: proliferasyon neuroblasts rostral göçmen akışı (RMS) aracılığıyla hareket göç büyük ölçüde, lateral ventriküllerin subependimal bölgesi (SEZ) oluşur ve yeni nöronlar ayırt etmek ve koku ampuller (OB) içinde entegre. Bu üç etki alanı biyolojik olayların her belirgin düzenleyen, hücresel, moleküler ve fizyolojik mekanizmaların çalışması için ideal bir platform olarak hizmet vermektedir. Bu yazıda, ekstraselüler koşulları yakından neuroblasts göç in vivo ortamda taklit postnatal beyin dokusu için optimize edilmiş bir Organotipik dilim testi açıklar. Biz tahlil, üniforma, odaklı ve hızlı hareket için RMS içinde neuroblasts sağladığını göstermektedir. Bu testte, farklı genetik geçmişleri fareler çapraz nakli yaklaşımlar kullanılarak nöronal göçün hücre özerk ve özerk olmayan bir yönetmelik çalışması için son derece uygun olacaktır.

Watch this Scientific Journal Video about An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain at JoVE.com

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Bu protokol, postnatal beyin ve rostral göçmen akışı neuroblast göç yüksek çözünürlüklü zaman atlamalı görüntüleme için optimize edilmiş bir Organotipik dilim tahlil açıklamaktadır.

Doğum Sonrası Beyin Nöronal Göç Yüksek Çözünürlüklü Zaman Aşımı Görüntüleme için bir Organotipik Dilim Testi

Neurogenesis in the postnatal brain depends on maintenance of three biological events: proliferation of progenitor cells, migration of neuroblasts, as ...

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Neuroscience

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

Nöronal Sakatlık incelenmesi için Organotipik Hipokampal Dilim Kültür Modeli

Organotypic hippocampal slice culture is an in vitro method to examine mechanisms of neuronal injury in which the basic architecture and composition of ...

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

Gelişmekte olan Zebra balığı önbeyin klonal olarak ilişkilidir Sinir Progenitör Hücreler Zaman atlamalı Canlı Görüntüleme

Precise patterns of division, migration and differentiation of neural progenitor cells are crucial for proper brain development and function1,2. To ...

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

Organotipik Kültürleri nöronal Çığ Çok elektrot Dizi Kayıtlar

The cortex is spontaneously active, even in the absence of any particular input or motor output.  During development, this activity is important for the ...

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor

Görüntüleme Nöronal cevaplar Vomeronasal Organ Dilim hazırlıklar Genetiği Kodlanmış Kalsiyum Sensör ifade

The vomeronasal organ (VNO) detects chemosensory signals that carry information about the social, sexual and reproductive status of the individuals within ...

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

İnsan orta beyin için yüksek çözünürlüklü Fonksiyonel Manyetik Rezonans Görüntüleme Yöntemleri

Functional MRI (fMRI) is a widely used tool for non-invasively measuring correlates of human brain activity. However, its use has mostly been focused upon ...

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

Yetişkin Fare önbeyin akut dilimleri içinde Neuroblast Göç Time-lapse Görüntüleme

There is a substantial body of evidence indicating that new functional neurons are constitutively generated from an endogenous pool of neural stem cells ...

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

Fare Akut Beyin Dilimleri Neuroblast Göç vivo Doğum Sonrası Elektroporasyonla ve Time-lapse Görüntüleme

The subventricular zone (SVZ) is one of the main neurogenic niches in the postnatal brain. Here, neural progenitors proliferate and give rise to ...

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Serebellar Granül Nöronlar Genetik Manipülasyon<em&gt; In Vitro</em&gt; Ve<em&gt; In Vivo</em&gt; Nöronal morfoloji ve Göç Eğitim için

Developmental events in the brain including neuronal morphogenesis and migration are highly orchestrated processes. In vitro and in vivo analyses allow ...

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

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Yüksek çözünürlük<em&gt; İn Vivo</em&gt; 3T Manyetik Rezonans Görüntüleme kullanma İnsan hipokampal alanlar için Manuel Segmentasyon Protokolü

The human hippocampus has been broadly studied in the context of memory and normal brain function and its role in different neuropsychiatric disorders has ...

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Embriyonik Fare Beyni Organotipik Dilim Kültüründe Nöronların Geçiş Sürekli Konfokal Görüntüleme Kullanımı<em&gt; Utero&#39;da</em&gt; Elektroporasyon

In utero electroporation is a rapid and powerful approach to study the process of radial migration in the cerebral cortex of developing mouse embryos. It ...