Name: application of light-cured dental adhesive resin for mounting electrodes or microdialysis probes in chronic experiments
Uploaded: 2007-07-30T00:00:00
Description: Watch this Scientific Journal Video about Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments at JoVE.com

RIKEN - BSI我们感谢我们的同事为他们的好心的支持和富有成效的意见，在此工作。我想滋森教授，博士，教授摹BOR灶火SZ表示衷心的感谢。 Kiyoji松山，卡塔琳AK缂丝和RP ð Dobolyi谁劝我在日本高级研究的研究生，研究生课程学习，微透析方法。最后，我们感谢克里斯先生Sarnecky Akitsugu高须幸雄博士在其支持视频录制。

第1步。制备探针植入<ol><li>麻醉（在视频中未显示） 麻醉interperitoneal一个氯胺酮注射液（12.5毫克/公斤体重）和甲苯噻嗪（25毫克/公斤）的混合物，手术麻醉产生一个稳定的水平每只鸟。 </li><li>修正了一个头，一个立体框架 使用Xylocaine（TM）果冻耳棒“的压力，以减少当地的阵痛 。 鸟头是固定在耳酒吧和喙的持有人，持有在45度角的?...

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	<li>Adell, A., Artigas, F., Boulton, A., Baker, G., Bateson, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+Brain+microdialysis:+Principles+and+applications.">In vivo Brain microdialysis: Principles and applications.</a> In Vivo Neuromethods: Neuromethods. 32, 325-357 (1998).</li><li>Kendrick, K., Robinson, T., Justice, J., Bateson, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microdialysis+in+large+unrestrained+animals:+neuroendocrine+and+behavioral+studies+of+acetylcholine,+amino+acid,+monoamine+and+neuropeptide+release+in+the+sheep.">Microdialysis in large unrestrained animals: neuroendocrine and behavioral studies of acetylcholine, amino acid, monoamine and neuropeptide release in the sheep.</a> Microdialysis in the Neuroscience. 7, 327-348 (1991).</li><li>Oakley, B., Schafer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Experimental Neurobiology: A laboratory Manual. , (1978).</li><li>Okumura, T., Yamashita, Y., Okanoya, K., Tani, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Function+of+the+sensori-motor+nucleus+NIf+in+generation+of+complex+syntactical+song+in+the+Bengalese+Finch+I.+A+Biological+study.">Function of the sensori-motor nucleus NIf in generation of complex syntactical song in the Bengalese Finch I. A Biological study.</a> Soc. Neurosci Abst. 33, (2007).</li><li>Boulton, A., Baker, G., Robinson, T., Bateson, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+relation+of+brain+electrical+activity+to+behavior.">The relation of brain electrical activity to behavior.</a> In Vivo Neuromethods: Neuromethods. 32, 325-357 (1998).</li><li>Yamashita, Y., Okumura, T., Okanoya, K., Tani, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Function+of+the+sensori-motor+nucleus+NIf+in+generation+of+complex+syntactical+song+in+the+Bengalese+finch.">Function of the sensori-motor nucleus NIf in generation of complex syntactical song in the Bengalese finch.</a> , 101-104 (2007).</li></ol>

引进技术相对于传统的自固化丙烯酸树脂的应用，有几个重要的优点： 1。直接键合： 可以附着到骨自固化丙烯酸一段时间，但它不会坚持到骨。因此，锚固螺丝需要修复安装基座（见图2a）。相比之下，光固化树脂可以直接让螺丝不需要骨（图2B）的债券。因此，安装基座的大小，可以大大减少。此外，光固化树脂还坚持某些金属，如果你用“金属底漆”。粘接可变材料的这一特点，使我们一个...

1A照片显示微透析探针（一），螺帽（b）和指导导管（C）。在这张照片中，引导导管牙科树脂坚持头骨直接包围。照片B显示了一鸟在药物灌注。在头顶上，有一个微透析探针连接到罚款tefron管。比例尺= 1厘米，分别为A和B的2厘米，。 <img src="http://www.jove.com/files/249_2008_8_26_3/249_Figure-01.png" /> 图1 -一个微透析探针（A）和鸟在药物灌注的照片（二 ）

application of light-cured dental adhesive resin for mounting electrodes or microdialysis probes in chronic experiments

在慢性录音实验中，自固化牙科的丙烯酸树脂已被用来作为一个电极或微透析探针的安装基座。由于这些丙烯酸骨不债券，螺丝已被用来作为锚。然而，雀或鼠标一样的小实验动物，他们的头盖骨是很脆弱的，并不能成功举办的基石。在这份报告中，我们提出了一个新的应用程序安装电极或微透析探针在慢性实验基地牙科光固化树脂。这种材料可直接粘接头盖骨。因此，锚钉不需要手术视野，可以大大减少。以往的经验表明，粘接效果保持2个月以上。传统的树脂的窗口时，材料柔韧性和可行的几分钟。然而，这些牙齿胶粘剂的工作时间的窗口，是显着更广泛和可调。

Watch this Scientific Journal Video about Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments at JoVE.com

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

在这份报告中，我们提出了一个新的应用程序安装电极或微透析探针在慢性实验基地牙科光固化树脂。这种材料可直接粘接头盖骨。

光固化牙科粘合树脂安装电极或微透析探针在慢性实验中的应用

In chronic recording experiments, self-curing dental acrylic resins have been used as a mounting base of electrodes or microdialysis-probes. Since these ...

Research

JoVE Journal

Biology

Preparation of Artificial Bilayers for Electrophysiology Experiments

电生理实验人工双层膜的制备

Planar lipid bilayers, also called artificial lipid bilayers, allow you to study ion-conducting channels in a well-defined environment. These bilayers can ...

科研

生物学

Fabrication of Amperometric Electrodes

安培电极的制备

Carbon fiber electrodes are crucial for the detection of catecholamine release from vesicles in single cells for amperometry measurements.  Here, we ...

Robust 3D DNA FISH Using Directly Labeled Probes

强大的3D直接标记的探针DNA鱼

3D DNA FISH has become a major tool for  analyzing three-dimensional organization of the nucleus, and several variations  of the technique have been ...

Facial Transplants in Xenopus laevis Embryos

Facial Transplants in Xenopus laevis Embryos

在面部移植<em&gt;非洲爪蟾</em&gt;胚胎

Craniofacial birth defects occur in 1 out of every 700 live births, but etiology is rarely known due to limited understanding of craniofacial development. ...

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

多层安装于斑马鱼的长期光表镜

Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is ...

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

从成年小鼠切牙牙源性上皮干细胞的分离和培养

Understanding the cellular and molecular mechanisms that underlie tooth regeneration and renewal has become a topic of great interest1-4, and the mouse ...

Dissection and Mounting of Drosophila Pupal Eye Discs

Dissection and Mounting of Drosophila Pupal Eye Discs

解剖和安装的<em&gt;果蝇</em&gt;蛹眼光盘

The Drosophila melanogaster eye disc is a powerful system that can be used to study many different biological processes.  It contains approximately 800 ...

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

大会和“剪切环”中的应用：一种新型内皮模型轨道，单向和定期流体流动和剪应力

Deviations from normal levels and patterns of vascular fluid shear play important roles in vascular physiology and pathophysiology by inducing adaptive as ...

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells

遗传编码的荧光一氧化氮的应用（NO•）探针，所述geNOps，用于在单细胞NO•信号的实时影像

Nitric Oxide (NO&#8226;) is a small radical, which mediates multiple important cellular functions in mammals, bacteria and plants. Despite the existence ...

Inducing Apical Periodontitis in Mice

诱导小鼠的牙周炎

The mechanisms involved in local induced inflammation can be studied using several available animal models. One of these is the induction of apical ...