Name: imaging cell shape change in living drosophila embryos
Uploaded: 2011-03-30T13:00:00
Description: Watch this Scientific Journal Video about Imaging Cell Shape Change in Living Drosophila Embryos at JoVE.com

우리는 기꺼이이 프로토콜을 개발한있는 기초를 제공 에릭 Wieschaus을 인정합니다. 우리 작품은 베르나 &amp; 마스 맥린 생화학 계열 분자 생물학 시작 - 업 상, 의학 베일러 대학에서 지원됩니다.

1. 엠브료 컬렉션 컵 조립 <ol><li> 가능한 한 매끄러운로 가장자리를 만들고 레이저로 100 ML 트라이 - 옥수수 비커의 바닥을 잘라. 이 절대적으로 필요한 것은 아니지만, 컵, 당신은 또한 상단에서 세 모서리를 장식하는 경우 처리하기가 쉽습니다. </li><li> 와이어 메쉬 (6cm X 6cm)의 정사각형을 잘라. 미리 예열 뜨거운 접시에, 내부 퓸 후드, 레이어 강력 알루미늄 호일의 조각 위에 와이어 메쉬의 광장....

<ol>
	<li>Sagona, A. P., Stenmark, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytokinesis+and+cancer.">Cytokinesis and cancer.</a> FEBS Lett. 584, 2652-2661 (2010).</li><li>Baum, B., Settleman, J., Quinlan, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transitions+between+epithelial+and+mesenchymal+states+in+development+and+disease.">Transitions between epithelial and mesenchymal states in development and disease.</a> Semin Cell Dev Biol. 19, 294-308 (2008).</li><li>Kibar, Z., Capra, V., Gros, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Toward+understanding+the+genetic+basis+of+neural+tube+defects.">Toward understanding the genetic basis of neural tube defects.</a> Clin Genet. 71, 295-310 (2007).</li><li>Jazwinska, A., Ribeiro, C., Affolter, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epithelial+tube+morphogenesis+during+Drosophila+tracheal+development+requires+Piopio,+a+luminal+ZP+protein.">Epithelial tube morphogenesis during Drosophila tracheal development requires Piopio, a luminal ZP protein.</a> Nat Cell Biol. 5, 895-901 (2003).</li><li>Martin, P., Parkhurst, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parallels+between+tissue+repair+and+embryo+morphogenesis.">Parallels between tissue repair and embryo morphogenesis.</a> Development. 131, 3021-3034 (2004).</li><li>Royou, A., Field, C., Sisson, J. C., Sullivan, W., Karess, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reassessing+the+role+and+dynamics+of+nonmuscle+myosin+II+during+furrow+formation+in+early+Drosophila+embryos.">Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos.</a> Mol Biol Cell. 15, 838-850 (2004).</li><li>Lecuit, T., Wieschaus, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polarized+insertion+of+new+membrane+from+a+cytoplasmic+reservoir+during+cleavage+of+the+Drosophila+embryo.">Polarized insertion of new membrane from a cytoplasmic reservoir during cleavage of the Drosophila embryo.</a> J Cell Biol. 150, 849-860 (2000).</li><li>Mavrakis, M., Rikhy, R., Lippincott-Schwartz, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Plasma+membrane+polarity+and+compartmentalization+are+established+before+cellularization+in+the+fly+embryo.">Plasma membrane polarity and compartmentalization are established before cellularization in the fly embryo.</a> Dev Cell. 16, 93-104 (2009).</li><li>Cao, J., Crest, J., Fasulo, B., Sullivan, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cortical+Actin+Dynamics+Facilitate+Early-Stage+Centrosome+Separation.">Cortical Actin Dynamics Facilitate Early-Stage Centrosome Separation.</a> Curr Biol. , (2010).</li><li>Cao, J., Albertson, R., Riggs, B., Field, C. M., Sullivan, W. N. u. f. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=a+Rab11+effector,+maintains+cytokinetic+furrow+integrity+by+promoting+local+actin+polymerization.">a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization.</a> J Cell Biol. 182, 301-313 (2008).</li><li>Sokac, A. M., Wieschaus, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+actin-dependent+endocytosis+is+zygotically+controlled+to+initiate+Drosophila+cellularization.">Local actin-dependent endocytosis is zygotically controlled to initiate Drosophila cellularization.</a> Dev Cell. 14, 775-786 (2008).</li><li>Fox, D. T., Peifer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Abelson+kinase+(Abl)+and+RhoGEF2+regulate+actin+organization+during+cell+constriction+in+Drosophila.">Abelson kinase (Abl) and RhoGEF2 regulate actin organization during cell constriction in Drosophila.</a> Development. 134, 567-578 (2007).</li><li>Martin, A. C., Kaschube, M., Wieschaus, E. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulsed+contractions+of+an+actin-myosin+network+drive+apical+constriction.">Pulsed contractions of an actin-myosin network drive apical constriction.</a> Nature. 457, 495-499 (2009).</li><li>Bertet, C., Sulak, L., Lecuit, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myosin-dependent+junction+remodelling+controls+planar+cell+intercalation+and+axis+elongation.">Myosin-dependent junction remodelling controls planar cell intercalation and axis elongation.</a> Nature. 429, 667-671 (2004).</li><li>Blankenship, J. T., Backovic, S. T., Sanny, J. S., Weitz, O., Zallen, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multicellular+rosette+formation+links+planar+cell+polarity+to+tissue+morphogenesis.">Multicellular rosette formation links planar cell polarity to tissue morphogenesis.</a> Dev Cell. 11, 459-470 (2006).</li><li>Caussinus, E., Colombelli, J., Affolter, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tip-cell+migration+controls+stalk-cell+intercalation+during+Drosophila+tracheal+tube+elongation.">Tip-cell migration controls stalk-cell intercalation during Drosophila tracheal tube elongation.</a> Curr Biol. 18, 1727-1734 (2008).</li><li>Gervais, L., Casanova, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+coupling+of+cell+elongation+and+lumen+formation+in+a+single+cell.">In vivo coupling of cell elongation and lumen formation in a single cell.</a> Curr Biol. 20, 359-366 (2010).</li><li>Gorfinkiel, N., Blanchard, G. B., Adams, R. J., Arias, M. a. r. t. i. n. e. z., A, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+control+of+global+cell+behaviour+during+dorsal+closure+in+Drosophila.">Mechanical control of global cell behaviour during dorsal closure in Drosophila.</a> Development. 136, 1889-1898 (2009).</li><li>Solon, J., Kaya-Copur, A., Colombelli, J., Brunner, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulsed+forces+timed+by+a+ratchet-like+mechanism+drive+directed+tissue+movement+during+dorsal+closure.">Pulsed forces timed by a ratchet-like mechanism drive directed tissue movement during dorsal closure.</a> Cell. 137, 1331-1342 (2009).</li><li>Bownes, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+photographic+study+of+development+in+the+living+embryo+of+Drosophila+melanogaster.">A photographic study of development in the living embryo of Drosophila melanogaster.</a> J Embryol Exp Morphol. 33, 789-801 (1975).</li><li>Sokac, A. M., Wieschaus, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Zygotically+controlled+F-actin+establishes+cortical+compartments+to+stabilize+furrows+during+Drosophila+cellularization.">Zygotically controlled F-actin establishes cortical compartments to stabilize furrows during Drosophila cellularization.</a> J Cell Sci. 121, 1815-1824 (2008).</li><li>Morin, X., Daneman, R., Zavortink, M., Chia, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+protein+trap+strategy+to+detect+GFP-tagged+proteins+expressed+from+their+endogenous+loci+in+Drosophila.">A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila.</a> Proc Natl Acad Sci U S A. 98, 15050-15055 (2001).</li><li>Oda, H., Tsukita, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+imaging+of+cell-cell+adherens+junctions+reveals+that+Drosophila+mesoderm+invagination+begins+with+two+phases+of+apical+constriction+of+cells.">Real-time imaging of cell-cell adherens junctions reveals that Drosophila mesoderm invagination begins with two phases of apical constriction of cells.</a> J Cell Sci. 114, 493-501 (2001).</li><li>Kiehart, D. P., Galbraith, C. G., Edwards, K. A., Rickoll, W. L., Montague, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiple+forces+contribute+to+cell+sheet+morphogenesis+for+dorsal+closure+in+Drosophila.">Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila.</a> J Cell Biol. 149, 471-490 (2000).</li></ol>

프로토콜은 여기에 개발 비행의 배아에서 세포 모양의 변화 다수의 라이브, 공촛점 이미징을 허용합니다 설명했다. 이미지에 대한 GFP 주식은 개별 연구소 (표 2)에 의해 준비하지만, 많은 이러한 주식은 인디애나 대학 (블루밍턴에서 같은 Drosophila 증권 센터로 센터에서 공개적으로 사용할 수있는 수 <a href="http://flystocks.bio.indiana.edu">http://flystocks.bio.indiana.edu</a> )과 파리통 예일 대학 (앳 증권 ...

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<tr>
<td>Slides</td>
<td>&nbsp;</td>
<td>Fisherbrand/Fisher Scientific</td>
<td>12-550-343</td>
<td>&nbsp;</td>
<tr>
<td>Cover slips 25x25</td>
<td>&nbsp;</td>
<td>Fisher Scientific (Corning #2865-25)</td>
<td>1 2-524C</td>
<td>&nbsp;</td>
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<td>Squirt bottles (H2O)</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>02-897-11</td>
<td>&nbsp;</td>
<tr>
<td>50 ml Falcon tubes</td>
<td>&nbsp;</td>
<td>Fisher Scientific (BD# 352070)</td>
<td>14-432-22</td>
<td>&nbsp;</td>
<tr>
<td>Bulbs for small pipets, 1 mL</td>
<td>&nbsp;</td>
<td>Fisherbrand/Fisher Scientific</td>
<td>03-448-21</td>
<td>&nbsp;</td>
<tr>
<td>Scintillation vials with caps</td>
<td>&nbsp;</td>
<td>VWR (Wheaton #986546)</td>
<td>66021-533</td>
<td>&nbsp;</td>
<tr>
<td>Tri-Corn Beakers, 100 mL</td>
<td>&nbsp;</td>
<td>Electron Microscopy Sciences</td>
<td>60970</td>
<td>&nbsp;</td>
<tr>
<td>BD Falcon Petri dish 60x15mm </td>
<td>&nbsp;</td>
<td>Fisher Scientific (BD# 351007)</td>
<td>08757 100B</td>
<td>&nbsp;</td>
<tr>
<td>BD Falcon Cell strainer</td>
<td>&nbsp;</td>
<td>Fisher Scientific (BD#352350)</td>
<td>08-771-2</td>
<td>&nbsp;</td>
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<td>Yellow pipet tips</td>
<td>&nbsp;</td>
<td>Ranin</td>
<td>L200</td>
<td>&nbsp;</td>
<tr>
<td>Stainless steel mesh, 304, 12x24</td>
<td>&nbsp;</td>
<td>Small Parts</td>
<td>CX-0150-F-01</td>
<td>&nbsp;</td>
<tr>
<td>Glass 5&frac34; inch Pasteur Pipets</td>
<td>&nbsp;</td>
<td>Fisherbrand/Fisher Scientific</td>
<td>13-678-20B</td>
<td>&nbsp;</td>
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<td>P4 Filter paper</td>
<td>&nbsp;</td>
<td>Fisherbrand/Fisher Scientific</td>
<td>09-803-6F</td>
<td>&nbsp;</td>
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<td>Rubber bands</td>
<td>&nbsp;</td>
<td>Office Max</td>
<td>A620645</td>
<td>&nbsp;</td>
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<td>Scotch double-sided tape, &frac12; inch</td>
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<td>Office Max</td>
<td>A8137DM-2</td>
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<td>Robert Simmons Expression paint brushes E85 round #2</td>
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<td>Jerry&#x2019;s Artarama</td>
<td>56460</td>
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<td>Dumont #5 Forceps High Precision Inox</td>
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<td>Electron Microscopy Services</td>
<td>72701-DZ</td>
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<td>Razor blades</td>
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<td>VWR (BD #214010)</td>
<td>55411-050</td>
<td>&nbsp;</td>
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<td>Halocarbon Oil 27</td>
<td>&nbsp;</td>
<td>Sigma-Aldrich</td>
<td>H 8773</td>
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<td>Heptane</td>
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<td>Fisher Chemical/Fisher Scientific</td>
<td>H360-1</td>
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<td>BD Bacto Agar</td>
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<td>VWR (BD# 214010)</td>
<td>90000-760</td>
<td>&nbsp;</td>
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<td>Sucrose</td>
<td>&nbsp;</td>
<td>Sigma-Aldrich</td>
<td>S7903</td>
<td>&nbsp;</td>
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<td>p-Hydroxybenzoic acid</td>
<td>&nbsp;</td>
<td>Sigma-Aldrich</td>
<td>H5501</td>
<td>&nbsp;</td>
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<td>Red Star Active Dry Yeast</td>
<td>&nbsp;</td>
<td>LeSaffre</td>
<td>15700</td>
<td>&nbsp;</td>
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<td>Paper towels, C-fold</td>
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<td>Kleenex</td>
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<td>Heavy duty aluminum foil</td>
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<td>Reynolds Wrap</td>
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<td>Bleach</td>
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<td>Austin&#x2019;s A-1 Commercial</td>
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<td>100% Apple juice</td>
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<td>Ocean Spray or Tree Top</td>
<td>&nbsp;</td>
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imaging cell shape change in living drosophila embryos

개발 Drosophila melanogaster의 배아는 공촛점 이미징 살고 매우 의무가 아르 셀 모양 변경 번호를 겪습. 파리에서 셀 모양 변경은 더 높은 유기체에있는 사람들에게 유사하며, 그들은 조직 morphogenesis 운전. 그래서, 많은 경우에 그들의 연구는 인간의 질병 (표 1) 1-5 이해에 대한 직접적인 의미가 있습니다. 하위 세포 규모에서 이러한 세포 형태의 변화는 유전자 발현의 신호 전달, 세포 극성, cytoskeletal 개조 및 멤브레인 인신 매매에 이르기까지 활동의 제품입니다. 따라서 Drosophila의 배아들은 조직 morphogenesis 관련된으로 세포 모양의 변화를 평가할 수있는 상황뿐만 아니라를 제공뿐만 아니라 모양 세포 그 하위 세포 활동을 연구하기 위해 완전히 생리 환경을 제공합니다. 프로토콜 이미지 cellularization라는 특정 세포 모양의 변화를 위해 설계되었습니다 여기에 설명되어 있습니다. Cellularization는 극적인 플라즈마 막 성장의 과정이며, 그것은 궁극적으로 세포 배반엽에 syncytial 배아를 변환합니다. mitotic주기 14 계면에서 즉, 플라즈마 막 동시에 기본 상피 세포의 시트를 생성하기 위해 ~ 6000 cortically 고정 핵의 각 주위 invaginates. 이전 제안을 카운터, cellularization는 마이 오신 - 2 수축성 6 주도 아니지만, 대신에 주로 내부 매장 7 막의 exocytosis에 의해 연료입니다. 따라서 cellularization는 cytokinesis 또는 가로 - 가느다란 관 (T - 가느다란 관) 근육의 morphogenesis로 플라즈마 막 invagination 또는 확장을 필요로 세포 모양의 변화 동안 멤브레인 인신 매매를 공부하고있는 훌륭한 시스템입니다. 이 프로토콜은 쉽게 파리 배아의 다른 세포 형태 변경의 이미지에 적용하고, 그러한 배아 컬렉션의 무대를 변경하거나, 특정 방향 (테이블에있는 배아를 탑재 &quot;배아 접착제&quot;를 사용하는 것과 약간의 adaptations을 필요로됩니다 1) 8-19. 모든 경우에 흐름은 기본적으로 (그림 1)과 동일합니다. 복제 및 Drosophila transgenesis에 대한 표준 방법은 그린 형광 단백질 (GFP) 또는 그 변종에 융합, 관심의 단백질을 표현 안정적인 비행 주식을 준비하는 데,이 파리들은 배아의 재생 소스를 제공하고 있습니다. 또는 형광 단백질 / 프로브 직접 간단 마이크로 사출 기술을 통해 90-10 플라이 배아에 도입하고 있습니다. 그런 다음, 발달 행사 및 몇 군데으로 세포 모양의 변화에​​ 따라 태아가 수집되고 해부 현미경의 형태로 개최하고, 마지막으로 위치 및 공촛점 현미경에서 시간 저속 영상에 대한 마운트.

Watch this Scientific Journal Video about Imaging Cell Shape Change in Living Drosophila Embryos at JoVE.com

Imaging Cell Shape Change in Living Drosophila Embryos

과일 파리, Drosophila melanogaster의 조기 개발이 잘 이미징 방식에 적합 세포 모양의 변화의 숫자에 의해 특징입니다. 이 문서 Drosophila의 배아 라이브 공촛점 이미징에 필요한 기본적인 도구와 방법을 설명하며, cellularization라는 세포 모양의 변화에​​ 초점을 맞출 것이다.

생활의 이미징 셀 모양 변경 Drosophila 태아

The developing Drosophila melanogaster embryo undergoes a number of cell shape changes that are highly amenable to live confocal imaging.  Cell shape ...

Research

JoVE Journal

Biology

Imaging Cell Shape Change in Living Drosophila Embryos

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos

Midgastrula 스테이지 Drosophila의 태아에서 문화의 연결 준비

This video illustrates the procedure for making primary neuronal cultures from midgastrula stage Drosophila embryos. The methods for collecting embryos ...

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생물학

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

라이브 zebrafish의 배아에서 두 광자의 axotomy하고 시간이 경과 공촛점 영상

Zebrafish have long been utilized to study the cellular and molecular mechanisms of development by time-lapse imaging of the living transparent embryo.  ...

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

의 준비 Drosophila</em공중 드롭 프로토콜을 사용하여 라이브 영상을위한> 태아

Green fluorescent protein (GFP)-based timelapse live-imaging is a powerful technique for studying the genetic regulation of dynamic processes such as ...

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

의 전자 현미경을위한 배아의 준비 Drosophila 태아의 심장 관

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

In-vivo Centrifugation of Drosophila Embryos

In-vivo Centrifugation of Drosophila Embryos

Drosophila의 배아들 중에서 - 생체내의 원심 분리

A major strategy for purifying and isolating different types of intracellular organelles is to separate them from each other based on differences in ...

Upright Imaging of Drosophila Embryos

Upright Imaging of Drosophila Embryos

의 입식 이미징 Drosophila 태아

Several well-known morphogenetic gradients and cellular movements occur along the dorsal/ventral axis of the Drosophila embryo. However, the current ...

Primary Cell Cultures from Drosophila Gastrula Embryos

Primary Cell Cultures from Drosophila Gastrula Embryos

에서 기본 셀 문화 Drosophila Gastrula의 태아

Here we describe a method for preparing and culturing primary cells dissociated from Drosophila gastrula embryos.   In brief, a large amount of staged ...

Isolation and Purification of Kinesin from Drosophila Embryos

Isolation and Purification of Kinesin from Drosophila Embryos

Kinesin의 분리 및 정제에서 Drosophila 태아

Motor proteins move cargos along microtubules, and transport them to specific sub-cellular locations. Because altered transport is suggested to underlie a ...

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

생활에서 보여주는 동적 Kinetochore 단백질 행동과 염색체 모션으로 Mitotic 검사 점 유학<em&gt; Drosophila</em&gt; Syncytial 태아

The spindle assembly checkpoint (SAC) mechanism is an active signal, which monitors the interaction between chromosome kinetochores and spindle ...

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

사멸 세포 간극의 이미지를하는 동안 라이브<em&gt; 초파리</em&gt; 배아

The  proper elimination of unwanted or aberrant cells through apoptosis and  subsequent phagocytosis (apoptotic cell clearance) is crucial for normal  ...