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09:01 min
March 16th, 2011
DOI :
10.3791/2505-v
Chapters
0:05
Title
1:38
Creation of a Mutant Library: Mutagenesis
3:23
Creation of a Mutant Library: Readout
4:03
Mutant Identification and Characterization: Construction of Gradient
5:06
Mutant Identification and Characterization: Stamp Transfer of Bacteria
6:27
Mutagenesis and Functional Selection Protocols: Representative Results
8:14
Conclusion
Summary
在这里,我们展示了一个简单的协议来创建一个给定的目标序列的随机突变体库。我们表明,这种方法,这是在大肠杆菌体内进行,如何可以再加上功能选择进化出新的酶活动。
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