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10.4K Views
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10:02 min
April 10th, 2011
DOI :
10.3791/2509-v
Chapters
0:05
Title
0:42
Fabrication of Glass Needles and Wire Needles
2:15
Calibration of the Intermediate and Working Glass Needles
3:42
Coating Needles with Attachment Factors
4:13
Equipment Set Up and Needles Alignment
6:15
Cell Attachment
7:19
Axon Extension and Data Analysis
8:13
Representative Results for Controlling Axonal Development
9:43
Conclusion
Summary
アプリケーションと2000から1000 microdyneの範囲内のニューロンに作用する力の直接測定は、校正済みのガラス針を用いて高精度で実現されています。この方法論は、軸索の開始、軸索張力、軸索伸長の速度、および力ベクトルを含む、軸索の開発のいくつかの側面を、制御および測定に使用することができます。
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