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33.4K Views
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10:47 min
February 21st, 2011
DOI :
10.3791/2513-v
Chapters
0:00
Title
1:15
Dissection of Mouse Tracheas
3:40
Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
9:44
Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
10:28
Conclusion
8:25
Application of Cigarette Smoke to Cultured Epithelial Cells
Summary
肺上皮細胞は、マウスの気道から分離し、差別化された呼吸上皮のモデルとしての気液界面で培養することができます。プロトコルは、分離培養し、この環境毒素への分子応答を研究するために、たばこの主流煙にこれらの細胞を露出させるための記述されています。
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