Name: use of a hanging-weight system for isolated renal artery occlusion
Uploaded: 2011-07-19T13:00:00
Duration: 7 min 54 s
Description: Watch this Scientific Journal Video about Use of a Hanging-weight System for Isolated Renal Artery Occlusion at JoVE.com

वर्तमान अध्ययन संयुक्त राज्य अमेरिका स्वास्थ्य R01 HL092188 अनुदान के राष्ट्रीय संस्थानों, R01 DK083385 और R01HL098294, और संज्ञाहरण शिक्षा और अनुसंधान के लिए फाउंडेशन (FAER) HKE को अनुदान, एक FAER JYD और DFG रिसर्च (ड्यूश Forschungsgemeinschaft) अनुदान द्वारा समर्थित थे एजी के लिए फैलोशिप (GR2121/1-1).

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ब्याज की कोई संघर्ष की घोषणा की.

संक्षेप में, वर्तमान अध्ययन वृक्क धमनी रोड़ा के लिए फांसी वजन एक प्रणाली का उपयोग कर एक murine मॉडल में गुर्दे ischemia के प्रदर्शन के लिए एक उपन्यास तकनीक का वर्णन करता है. वास्तव में, इस अध्ययन अत्यधिक प्रतिलिपि प्रस्तुत करने योग्य चोट को दर्शाता है, इस प्रकार गुर्दे की डंडी की clamping के साथ जुड़े परिवर्तनशीलता कम से कम.

<table border="1"><tbody><tr><th> अभिकर्मक के नाम </th><th> कंपनी </th><th> सूचीपत्र संख्या </th><th> टिप्पणियाँ </th></tr><tr><td> Pentobarbital सोडियम </td><td> Vortech फार्मास्युटिकल रास, लिमिटेड </td><td> 9372 VPL </td><td> खारा में 4mg/mL </td></tr><tr><td> सीवन, रेशम 4.0 </td><td> हार्वर्ड उपकरण </td><td> 517698 </td><td></td></tr><tr><td> सीवन, 8.0 Prolene </td><td> Ethicon, संयुक्त राज्य अमरीका </td><td> M8739 </td><td> पुन: प्रयोज्य </td></tr><tr><td> विदारक माइक्रोस्कोप </td><td> Leica </td><td> n / a </td><td> उदार काम कर दूरी पर विचार </td></tr><tr><td> ताप टेबल </td><td> आर टी, Effenberger, जर्मनी </td><td> n / a </td><td> केवल और एकल प्रदाता </td></tr><tr><td> सर्जिकल उपकरणों </td><td> थोक मूल्य सूचकांक, Dumont </td><td></td><td> एक ठीक microvessel कैंची </td></tr></tbody></table>

use of a hanging-weight system for isolated renal artery occlusion

In hospitalized patients, over 50% of cases of acute kidney injury (AKI) are caused by renal ischemia 1-3. A recent study of hospitalized patients revealed that only a mild increase in serum creatinine levels (0.3 to 0.4 mg/dl) is associated with a 70% greater risk of death than in persons without any increase 1. Along these lines, surgical procedures requiring cross-clamping of the aorta and renal vessels are associated with a renal failure rates of up to 30% 4. Similarly, AKI after cardiac surgery occurs in over 10% of patients under normal circumstances and is associated with dramatic increases in mortality. AKI are also common complications after liver transplantation. At least 8-17% of patients end up requiring renal replacement therapy 5. Moreover, delayed graft function due to tubule cell injury during kidney transplantation is frequently related to ischemia-associated AKI 6. Moreover, AKI occurs in approximately 20% of patients suffering from sepsis 6.The occurrence of AKI is associated with dramatic increases of morbidity and mortality 1. Therapeutic approaches are very limited and the majority of interventional trials in AKI have failed in humans. Therefore, additional therapeutic modalities to prevent renal injury from ischemia are urgently needed 3, 7-9. 
To elucidate mechanisms of renal injury due to ischemia and possible therapeutic strategies murine models are intensively required 7-13. Mouse models provide the possibility of utilizing different genetic models including gene-targeted mice and tissue specific gene-targeted mice (cre-flox system). However, murine renal ischemia is technically challenging and experimental details significantly influence results. We performed a systematic evaluation of a novel model for isolated renal artery occlusion in mice, which specifically avoids the use of clamping or suturing the renal pedicle 14. This model requires a nephrectomy of the right kidney since ischemia can be only performed in one kidney due to the experimental setting. In fact, by using a hanging-weight system, the renal artery is only instrumented once throughout the surgical procedure. In addition, no venous or urethral obstruction occurs with this technique. We could demonstrate time-dose-dependent and highly reproducible renal injury with ischemia by measuring serum creatinine. Moreover, when comparing this new model with conventional clamping of the whole pedicle, renal protection by ischemic preconditioning is more profound and more reliable. Therefore his new technique might be useful for other researchers who are working in the field of acute kidney injury.

Watch this Scientific Journal Video about Use of a Hanging-weight System for Isolated Renal Artery Occlusion at JoVE.com

Use of a Hanging-weight System for Isolated Renal Artery Occlusion

तीव्र गुर्दे की चोट के लिए एक सटीक murine मॉडल (अकी) ischemia के कारण तीव्र गुर्दे चोट की जांच और संभवतः चिकित्सकीय उपकरण के लिए गुर्दे की चोट का इलाज खोजने के एक महत्वपूर्ण उपकरण है. फांसी वजन प्रणाली गुर्दे की भीड़ के कारण के बिना तत्काल और विश्वसनीय गुर्दे धमनी रोड़ा और reperfusion के लिए एक उपकरण प्रदान करता है.

पृथक गुर्दे धमनी आड़ के लिए एक हैंगिंग वजन सिस्टम का प्रयोग

In hospitalized patients, over 50% of cases of acute kidney injury (AKI) are caused by renal ischemia 1-3. A recent study of hospitalized patients ...

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