Name: use of a hanging-weight system for isolated renal artery occlusion
Uploaded: 2011-07-19T13:00:00
Duration: 7 min 54 s
Description: Watch this Scientific Journal Video about Use of a Hanging-weight System for Isolated Renal Artery Occlusion at JoVE.com

Настоящего исследования были поддержаны США Национальных Институтов Здоровья грант R01-HL092188, R01-DK083385 и R01HL098294 и фонда образования Анестезия и исследований (FAER) Гранты HKE, FAER предоставить JYD и DFG (Deutsche Forschungsgemeinschaft) Научные исследования стипендий (GR2121/1-1) для AG.

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Нет конфликта интересов объявлены.

Таким образом, настоящее исследование описывает новый метод для выполнения ишемии почек в мышиной модели с использованием подвесной-весовой системы для почечной окклюзии артерии. По сути, это исследование показывает, высокой воспроизводимостью травмы, таким образом минимизируя изменчивость связана с зажим почечной ножки.

<table border="1"><tbody><tr><th> Название реагента </th><th> Компания </th><th> Номер в каталоге </th><th> Комментарии </th></tr><tr><td> Натрий пентобарбитал </td><td> Vortech Фармацевтическая Ls, ООО </td><td> VPL 9372 </td><td> 4mg/mL в физиологическом растворе </td></tr><tr><td> Шовный, шелк 4,0 </td><td> Гарвардский аппарата </td><td> 517698 </td><td></td></tr><tr><td> Шовный, Prolene 8,0 </td><td> Ethicon, США </td><td> M8739 </td><td> многоразовый </td></tr><tr><td> препаровальная лупа </td><td> Leica </td><td> н / </td><td> рассмотреть щедрые рабочее расстояние </td></tr><tr><td> Отопление таблице </td><td> Rt, Effenberger, Германия </td><td> н / </td><td> только и одного поставщика </td></tr><tr><td> Хирургические инструменты </td><td> WPI, Дюмон </td><td></td><td> штрафа ножницеобразный микрососудов </td></tr></tbody></table>

use of a hanging-weight system for isolated renal artery occlusion

In hospitalized patients, over 50% of cases of acute kidney injury (AKI) are caused by renal ischemia 1-3. A recent study of hospitalized patients revealed that only a mild increase in serum creatinine levels (0.3 to 0.4 mg/dl) is associated with a 70% greater risk of death than in persons without any increase 1. Along these lines, surgical procedures requiring cross-clamping of the aorta and renal vessels are associated with a renal failure rates of up to 30% 4. Similarly, AKI after cardiac surgery occurs in over 10% of patients under normal circumstances and is associated with dramatic increases in mortality. AKI are also common complications after liver transplantation. At least 8-17% of patients end up requiring renal replacement therapy 5. Moreover, delayed graft function due to tubule cell injury during kidney transplantation is frequently related to ischemia-associated AKI 6. Moreover, AKI occurs in approximately 20% of patients suffering from sepsis 6.The occurrence of AKI is associated with dramatic increases of morbidity and mortality 1. Therapeutic approaches are very limited and the majority of interventional trials in AKI have failed in humans. Therefore, additional therapeutic modalities to prevent renal injury from ischemia are urgently needed 3, 7-9. 
To elucidate mechanisms of renal injury due to ischemia and possible therapeutic strategies murine models are intensively required 7-13. Mouse models provide the possibility of utilizing different genetic models including gene-targeted mice and tissue specific gene-targeted mice (cre-flox system). However, murine renal ischemia is technically challenging and experimental details significantly influence results. We performed a systematic evaluation of a novel model for isolated renal artery occlusion in mice, which specifically avoids the use of clamping or suturing the renal pedicle 14. This model requires a nephrectomy of the right kidney since ischemia can be only performed in one kidney due to the experimental setting. In fact, by using a hanging-weight system, the renal artery is only instrumented once throughout the surgical procedure. In addition, no venous or urethral obstruction occurs with this technique. We could demonstrate time-dose-dependent and highly reproducible renal injury with ischemia by measuring serum creatinine. Moreover, when comparing this new model with conventional clamping of the whole pedicle, renal protection by ischemic preconditioning is more profound and more reliable. Therefore his new technique might be useful for other researchers who are working in the field of acute kidney injury.

Watch this Scientific Journal Video about Use of a Hanging-weight System for Isolated Renal Artery Occlusion at JoVE.com

Use of a Hanging-weight System for Isolated Renal Artery Occlusion

Точное мышиной моделью острого повреждения почек (АКИ) в связи с ишемией является важным инструментом для исследования острого повреждения почек и, возможно, найти терапевтических средств для лечения почечных повреждений. Висит вес система предлагает инструмент для немедленного и надежного почечной окклюзии артерии и реперфузии, не вызывая почечную заторов.

Использование Висячие вес системы для изолированного почечной артерии Окклюзия

In hospitalized patients, over 50% of cases of acute kidney injury (AKI) are caused by renal ischemia 1-3. A recent study of hospitalized patients ...

Research

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Medicine

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Use of a Hanging Weight System for Coronary Artery Occlusion in Mice

Использование Висячие Вес системы для коронарной окклюзии артерии у мышей

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Acute liver injury due to ischemia can occur during several clinical procedures e.g. liver transplantation, hepatic tumor resection or trauma repair and ...

Mouse Model of Middle Cerebral Artery Occlusion

Мышь модели окклюзии средней мозговой артерии

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

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Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

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The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

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Stroke is a leading cause of death worldwide and continues to be one of the major causes of long-term adult disabilities. About 87% of strokes are ...

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Окклюзии средней мозговой артерии, позволяя реперфузии через ремонт общей сонной артерии у мышей

The ischemic stroke is a major cause of adult long-term disability and death worldwide. The current treatments available are limited, with only tissue ...

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