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15.6K Views
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04:50 min
June 17th, 2011
DOI :
10.3791/2599-v
Chapters
0:05
Title
1:17
Overview of Stage-top Incubator for Long-term Neuronal Imaging
1:46
Application of the Membrane Lid to the Culture Dish and Placement in the Incubator
2:19
Live Imaging of Mitochondria in Hippocampal Neurons
3:44
The Effects of Neuromodulation on Mitochondrial Movement in Cultured Hippocampal Neurons
4:27
Conclusion
Summary
我们描述了一个协议,允许在较长的时间,通过荧光显微镜观察生活中的神经元线粒体成像。过长时间的成像是通过一个mitochondrially有针对性的荧光蛋白,并利用一种廉价的舞台上方的孵化器,在我们的实验室设计和内置的慢病毒介导的表达。
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