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DOI :
10.3791/2662-v
March 1st, 2011
Chapters
0:05
Title
1:29
Collection of Whole Blood
2:02
Preparation of Whole Blood
3:53
Stimulation of the Whole Blood
5:15
Cytokine Assay
5:34
Over-Production of IL-1B in Whole Blood from Patients with Autoinflammatory Manifestations in Response to Stimulation with LPS in the Presence of ATP
6:08
Conclusion
我々は、自己炎症表現型を呈する患者では、このようなIL - 1βの産生などの炎症性サイトカインの産生を測定するための単純なイムノアッセイについて説明します。病原体関連分子パターンと全血培養では細胞を活性化することによって、特にリポ多糖で、サイトカインの分泌は、便利な全血上清で評価することができます。
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