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19:41 min
August 24th, 2007
DOI :
10.3791/267-v
Chapters
12:47
Day 3: Washing, secondary Ab incubation, nuclear stain, and mountin
0:07
Introduction
0:57
Day 1: Preparing German Glass Coverslips and Seeding Cells
3:16
Day 2: Fixing, Permeablilization, and Adding Primary Ab to Cells
0:00
Title
18:53
Conclusion
Summary
免疫細胞が存在、細胞内局在性、および培養細胞における目的の抗原の相対量を決定する強力な方法です。このプロトコルは1つの抗体を節約し、自分の染色を最大限に活用することを可能にする手順のわかりやすいシリーズを紹介。
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