Name: solid plate-based dietary restriction in caenorhabditis elegans
Uploaded: 2011-05-28T14:00:00
Description: Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

这项工作是支持到A - LH从弥敦道冲击中心（P30 AG13283）和授予R01（R01 AG028516）从国家老化研究所（NIA）的试点奖

<html> 1。细菌浓度的估计建立校准曲线本节描述为任何给定的菌种（如OP50，HT115），将作为食物来源C.限制饮食实验使用细菌浓度的估算方法线虫 。每一株用于细菌，应建立单独的校准曲线。 <ol><li>接种单菌落的E.大肠杆菌 OP50细菌捡到3毫升的液体LB培养基中的细菌从一个新鲜的LB琼脂连胜。 </li><li>广场OP50文化在37℃摇床，并允许细菌的生长过夜。 </li><li>...

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饮食限制（DR）是已知的，以增加寿命和延迟在广泛的物种，包括哺乳动物5-7中各种与年龄有关的的疾病的发病。已用于环境和遗传操作模式在 C DR 线虫，包括在定义一个半液体介质中8,9，稀释10-12日在液体介质中的细菌的食物来源和使用行为的突变体，饲料以较慢的速率13培养蠕虫。然而，DR的遗传模拟可能产生的表型无关的DR，而在液体培养基中培?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>羧</td><td> Duchefa Biochemie </td><td> C0109.25 </td><td>溶于水。 </td></tr><tr><td>卡那霉素</td><td>罗氏公司</td><td> 10106801001 </td><td>溶于水。 </td></tr><tr><td>细菌用的蛋白胨</td><td>流式细胞，Dickinson公司</td><td> DF0118072 </td><td></td></tr><tr><td>细菌用的蛋白胨</td><td>流式细胞，Dickinson公司</td><td> DF0123075 </td><td></td></tr><tr><td>细菌用的酵母提取物</td><td>流式细胞，Dickinson公司</td><td> DF0127071 </td><td></td></tr><tr><td>洋菜</td><td>流式细胞，Dickinson公司</td><td> DF00145170 </td><td></td></tr><tr><td>无菌培养管</td><td>美国科学</td><td> 1485-0810 </td><td></td></tr><tr><td>分光光度计</td><td> Amersham公司生物科学</td><td>基因定量亲</td><td> Amersham公司是通用电气医疗集团的一部分。 </td></tr><tr><td>解剖显微镜</td><td>蔡司</td><td> STEMI 2000 </td><td></td></tr><tr><td>离心分离</td><td> Beckman Coulter公司</td><td> Accuspin FR </td><td></td></tr></tbody></table>

solid plate-based dietary restriction in caenorhabditis elegans

没有营养不良或饥饿，减少食物的摄入，增加寿命和延迟发病的各种与年龄有关的疾病，在广泛的物种，包括哺乳动物。这也导致在体重和生育能力下降，以及血浆葡萄糖，胰岛素和IGF - 1在这些动物的较低水平。这种治疗方法通常被称为饮食限制（DR）或热量限制（CR）。 线虫已经成为一个重要的模式生物，研究衰老的生物学。环境和遗传操作已被用于模型的DR，并已显示出在 C的寿命延长线虫 。但是，许多报道DR 在 C的研究线虫是通过在液体介质中传播的动物，而在老龄问题领域的遗传研究的最标准在固体琼脂培养皿板。在这里，我们目前使用标准的固体NGM杀害细菌的琼脂板DR的协议。

Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

在这里，我们目前执行固体板为主的饮食限制在一个协议<em&gt; C。线虫</em&gt;与杀害细菌。

实心板为基础的饮食限制在线虫</em

Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

Research

JoVE Journal

Biology

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

测量线虫固体介质的寿命

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

科研

生物学

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

分离和<em在体外激活线虫精子

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

时间推移显微镜早期胚胎发育线虫

Caenorhabditis elegans has often been used as a model system in studies of early developmental processes.  The transparency of the 
embryos, the genetic ...

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Basic Caenorhabditis elegans Methods: Synchronization and Observation

基本的<em&gt;线虫</em&gt;方法：同步和观察

Research into the molecular and developmental biology of the nematode Caenorhabditis elegans was begun in the early seventies by Sydney Brenner and it has ...

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

前列腺素的提取与分析<em&gt;线虫</em

Caenorhabditis elegans is emerging as a powerful animal model to study the biology of lipids1-9. Prostaglandins are an important class of eicosanoids, ...

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

多不饱和脂肪酸的膳食补充剂<em&gt;秀丽隐杆线虫</em

Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, ...

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

栽培<em&gt;秀丽隐杆线虫</em&gt;在三个维度实验室

The use of genetic model organisms such as Caenorhabditis elegans has led to seminal discoveries in biology over the last five decades. Most of what we ...

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

线虫的耗氧率测定

Optimal mitochondrial function is critical for healthy cellular activity, particularly in cells that have high energy demands like those in the nervous ...

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

量化秀丽隐杆线虫中组织特异性蛋白静剂下降

The ability to maintain proper function and folding of the proteome (protein homeostasis) declines during normal aging, facilitating the onset of a ...

Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

自动化秀丽隐杆线虫的聚集定量

A rise in the prevalence of neurodegenerative protein conformational diseases (PCDs) has fostered a great interest in this subject over the years. This ...