Name: solid plate-based dietary restriction in caenorhabditis elegans
Uploaded: 2011-05-28T14:00:00
Description: Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Ce travail a été soutenu par une bourse de pilote de Centre de choc Nathan (P30 AG13283) et une subvention R01 (R01 AG028516) du National Institute on Aging (NIA) à A.-LH

<html> 1. Établir une courbe d&#39;étalonnage pour l&#39;estimation des concentrations de bactéries Cette section décrit une méthode pour l&#39;estimation de la concentration de bactéries pour toute souche donnée de bactéries (par exemple, OP50, HT115) qui sera utilisé comme source de nourriture pour les expériences de restriction alimentaire en C. elegans. Courbes d&#39;étalonnage distincts devraient être établis pour chaque bactérie souche utilisée. <ol><li> Inocul...

<ol>
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La restriction alimentaire (DR) est connu pour augmenter la durée de vie et de retarder l&#39;apparition de diverses maladies liées au vieillissement dans une large gamme d&#39;espèces, y compris les mammifères 5-7. Manipulations environnementaux et génétiques ont été utilisées pour modéliser DR en C. elegans, y compris les vers de culture dans un milieu semi-défini de liquide 8,9, la dilution de la source de nourriture des bactéries en milieu liquide 10-12, et l&#39...

<table border="1"><tbody><tr><td> Nom du réactif </td><td> Société </td><td> Numéro de catalogue </td><td> Commentaires </td></tr><tr><td> Carbénicilline </td><td> Duchefa Biochemie </td><td> C0109.25 </td><td> Dissous dans l&#39;eau. </td></tr><tr><td> Kanamycine </td><td> Roche </td><td> 10106801001 </td><td> Dissous dans l&#39;eau. </td></tr><tr><td> Bacto-peptone </td><td> Becton, Dickinson and Company </td><td> DF0118072 </td><td></td></tr><tr><td> Bacto-tryptone </td><td> Becton, Dickinson and Company </td><td> DF0123075 </td><td></td></tr><tr><td> Bacto-extrait de levure </td><td> Becton, Dickinson and Company </td><td> DF0127071 </td><td></td></tr><tr><td> Agar </td><td> Becton, Dickinson and Company </td><td> DF00145170 </td><td></td></tr><tr><td> Tube de culture stérile </td><td> USA Scientific </td><td> 1485-0810 </td><td></td></tr><tr><td> Spectrophotomètre </td><td> Amersham Biosciences </td><td> Gene Quant Pro </td><td> Amersham fait maintenant partie de GE Healthcare. </td></tr><tr><td> Microscope à dissection </td><td> Zeiss </td><td> Stemi 2000 </td><td></td></tr><tr><td> Centrifugeuse </td><td> Beckman Coulter </td><td> Accuspin FR </td><td></td></tr></tbody></table>

solid plate-based dietary restriction in caenorhabditis elegans

Réduction de la consommation d&#39;aliments sans malnutrition ou de famine est connue pour augmenter la durée de vie et de retarder l&#39;apparition de diverses maladies liées au vieillissement dans une large gamme d&#39;espèces, y compris les mammifères. Elle entraîne également une diminution du poids corporel et de la fertilité, ainsi que des niveaux plus bas de glucose plasmatique, l&#39;insuline et l&#39;IGF-1 chez ces animaux. Ce traitement est souvent désigné comme la restriction alimentaire (DR) ou de la restriction calorique (RC). Le nématode Caenorhabditis elegans est apparu comme un organisme modèle important pour étudier la biologie du vieillissement. Manipulations environnementaux et génétiques ont été utilisées pour modéliser DR et ont montré de prolonger la durée de vie en C. elegans. Cependant, la plupart des études ont rapporté DR en C. elegans ont été réalisées en propageant des animaux dans des milieux liquides, tandis que la plupart des études génétiques dans le domaine du vieillissement ont été réalisés sur la gélose standard de solide dans des boîtes de Pétri. Nous présentons ici un protocole standard de DR à l&#39;aide NGM solides à base de gélose plaque avec des bactéries tuées.

Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Nous présentons ici un protocole pour la réalisation solide plaque de base de la restriction alimentaire au<em&gt; C. elegans</em&gt; Avec des bactéries tuées.

Solide plaque à base de restriction alimentaire Caenorhabditis elegans</em

Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

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Biology

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

Mesure Caenorhabditis elegans</emDurée de vie> sur des milieux solides

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

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Biologie

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

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Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

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Time-lapse de microscopie du début de l&#39;embryogenèse dans les Caenorhabditis elegans

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De base<em&gt; Caenorhabditis elegans</em&gt; Méthodes: Synchronisation et d&#39;observation

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Prostaglandin Extraction and Analysis in Caenorhabditis elegans

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Extraction de prostaglandine et de l&#39;analyse en<em&gt; Caenorhabditis elegans</em

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Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

La supplémentation alimentaire en acides gras polyinsaturés dans<em&gt; Caenorhabditis elegans</em

Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, ...

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la culture de<em&gt; Caenorhabditis elegans</em&gt; En trois dimensions dans le laboratoire

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Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

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Mesure des taux de consommation d’oxygène intactes de Caenorhabditis elegans

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The ability to maintain proper function and folding of the proteome (protein homeostasis) declines during normal aging, facilitating the onset of a ...

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modélisation des maladies neurodégénératives associées à l’âge à Caenorhabditis elegans

Battling human neurodegenerative pathologies and managing their pervasive socioeconomic impact is becoming a global priority. Notwithstanding their ...