Name: solid plate-based dietary restriction in caenorhabditis elegans
Uploaded: 2011-05-28T14:00:00
Description: Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

この作品は、A. - LHへネイサンショックセンター（P30 AG13283）と国立老化研究所（NIA）からR01助成金（R01 AG028516）からのパイロット賞によってサポートされていました

<html> 1。細菌の濃度を推定するための検量線の確立このセクションでは、C言語の食事制限の実験のための食料源として使用される任意の細菌株（例えば、OP50、HT115）のための細菌の濃度を推定する方法を説明しますエレガンス 。別々の検量線は、株が使用されている各細菌のために確立されるべきである。 <ol><li> E.のシングルコロニーを植菌大?...

<ol>
	<li>Hsu, A. L., Murphy, C. T., Kenyon, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+aging+and+age-related+disease+by+DAF-16+and+heat-shock+factor.">Regulation of aging and age-related disease by DAF-16 and heat-shock factor.</a> Science. 300, 1142-1145 (2003).</li><li>Kenyon, C., Chang, J., Gensch, E., Rudner, A., Tabtiang, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+C.+elegans+mutant+that+lives+twice+as+long+as+wild+type.">A C. elegans mutant that lives twice as long as wild type.</a> Nature. 366, 461-464 (1993).</li><li>Apfeld, J., Kenyon, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+lifespan+by+sensory+perception+in+Caenorhabditis+elegans.">Regulation of lifespan by sensory perception in Caenorhabditis elegans.</a> Nature. 402, 804-809 (1999).</li><li>Ching, T. T., Paal, A. B., Mehta, A., Zhong, L., Hsu, A. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=drr-2+encodes+an+eIF4H+that+acts+downstream+of+TOR+in+diet-restriction-induced+longevity+of+C.+elegans.">drr-2 encodes an eIF4H that acts downstream of TOR in diet-restriction-induced longevity of C. elegans.</a> Aging Cell. 9, 545-557 (2010).</li><li>Weindruch, R., Walford, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Retardation of Aging and Disease by Dietary Restriction. , (1988).</li><li>Masoro, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Subfield+history:+caloric+restriction,+slowing+aging,+and+extending+life.">Subfield history: caloric restriction, slowing aging, and extending life.</a> Sci Aging Knowledge Environ. , RE2-RE2 (2003).</li><li>Masoro, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Overview+of+caloric+restriction+and+ageing.">Overview of caloric restriction and ageing.</a> Mech Ageing Dev. 126, 913-922 (2005).</li><li>Houthoofd, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Axenic+growth+up-regulates+mass-specific+metabolic+rate,+stress+resistance,+and+extends+life+span+in+Caenorhabditis+elegans.">Axenic growth up-regulates mass-specific metabolic rate, stress resistance, and extends life span in Caenorhabditis elegans.</a> Exp Gerontol. 37, 1371-1378 (2002).</li><li>Houthoofd, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=No+reduction+of+metabolic+rate+in+food+restricted+Caenorhabditis+elegans.">No reduction of metabolic rate in food restricted Caenorhabditis elegans.</a> Exp Gerontol. 37, 1359-1369 (2002).</li><li>Klass, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aging+in+the+nematode+Caenorhabditis+elegans:+major+biological+and+environmental+factors+influencing+life+span.">Aging in the nematode Caenorhabditis elegans: major biological and environmental factors influencing life span.</a> Mech Ageing Dev. 6, 413-429 (1977).</li><li>Bishop, N. A., Guarente, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two+neurons+mediate+diet-restriction-induced+longevity+in+C.+elegans.">Two neurons mediate diet-restriction-induced longevity in C. elegans.</a> Nature. 447, 545-549 (2007).</li><li>Panowski, S. H., Wolff, S., Aguilaniu, H., Durieux, J., Dillin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PHA-4/Foxa+mediates+diet-restriction-induced+longevity+of+C.+elegans.">PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans.</a> Nature. 447, 550-555 (2007).</li><li>Lakowski, B., Hekimi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+genetics+of+caloric+restriction+in+Caenorhabditis+elegans.">The genetics of caloric restriction in Caenorhabditis elegans.</a> Proc Natl Acad Sci U S A. 95, 13091-13096 (1998).</li><li>Kaeberlein, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lifespan+extension+in+Caenorhabditis+elegans+by+complete+removal+of+food.">Lifespan extension in Caenorhabditis elegans by complete removal of food.</a> Aging Cell. 5, 487-494 (2006).</li><li>Greer, E. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+AMPK-FOXO+pathway+mediates+longevity+induced+by+a+novel+method+of+dietary+restriction+in+C.+elegans.">An AMPK-FOXO pathway mediates longevity induced by a novel method of dietary restriction in C. elegans.</a> Curr Biol. 17, 1646-1656 (2007).</li></ol>

食事制限（DR）が寿命を増やすおよび5-7哺乳類を含む種の広い範囲で様々な加齢に伴う病気の発症を遅らせることが知られている。両方の環境と遺伝子操作は、CのモデルDRに使用されている半定義された液体培地8,9、液体培地10-12で細菌の食物源の希釈、およびより遅い速度13で送り、その動作の変異体の使用で培養型ワームなど、 エレガンス、。</e...

<table border="1"><tbody><tr><td> 試薬の名前 </td><td> 会社 </td><td> カタログ番号 </td><td> コメント </td></tr><tr><td>カルベニシリン</td><td> Duchefa Biochemie </td><td> C0109.25 </td><td>水に溶解。 </td></tr><tr><td>カナマイシン</td><td>ロッシュ</td><td> 10106801001 </td><td>水に溶解。 </td></tr><tr><td>バクトペプトン</td><td>ベクトンディッキンソン社</td><td> DF0118072 </td><td></td></tr><tr><td>バクトトリプトン</td><td>ベクトンディッキンソン社</td><td> DF0123075 </td><td></td></tr><tr><td>バクト酵母エキス</td><td>ベクトンディッキンソン社</td><td> DF0127071 </td><td></td></tr><tr><td>寒天</td><td>ベクトンディッキンソン社</td><td> DF00145170 </td><td></td></tr><tr><td>無菌培養管</td><td>米国サイエンティフィック</td><td> 1485-0810 </td><td></td></tr><tr><td>分光光度計</td><td>アマシャムバイオサイエンス</td><td>遺伝子クワントプロ</td><td>アマシャムは現在GEヘルスケアの一部です。 </td></tr><tr><td>解剖顕微鏡</td><td>ツァイス</td><td> STEMI 2000 </td><td></td></tr><tr><td>遠心</td><td>ベックマンコールター</td><td> Accuspin FR </td><td></td></tr></tbody></table>

solid plate-based dietary restriction in caenorhabditis elegans

栄養失調や飢餓のない食物摂取の減少は、寿命を向上させ、哺乳動物を含めた種の広い範囲で様々な加齢に伴う病気の発症を遅らせることが知られている。また、体重と生殖能力の低下だけでなく、血漿グルコース、インスリン、およびこれらの動物におけるIGF - 1の低いレベルになります。この治療はしばしば、食事制限（DR）やカロリー制限（CR）と呼ばれています。線虫Caenorhabditis elegansは老化の生物学を研究するための重要なモデル生物として注目されています。環境と遺伝子操作モデルDRに使用されてきたとCで寿命を延ばすことが示されている両方エレガンス 。しかし、C言語で報告されたDRの研究の多くエレガンスは、加齢分野における遺伝学的研究のほとんどはペトリ皿の標準固形寒天上で行われた一方、液体培地で動物を伝播することによって行われた。ここでは、死菌と標準固体NGM寒天ベースのプレートを使用してDRのプロトコルを提示する。

Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

ここでは、固体プレートベースの食事制限を行うためのプロトコルを提示<em&gt; C.エレガンス</em&gt;死菌で。

固体プレートベースの食餌制限線虫（Caenorhabditis elegans）</em

Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

Research

JoVE Journal

Biology

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

計測線虫（Caenorhabditis elegans）</em固体培地上>寿命

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

生物学

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

アイソレーションと試験管内で</emの>活性化線虫（Caenorhabditis elegans）精子

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

の初期発生のタイムラプス顕微鏡線虫（Caenorhabditis elegans）

Caenorhabditis elegans has often been used as a model system in studies of early developmental processes.  The transparency of the 
embryos, the genetic ...

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Basic Caenorhabditis elegans Methods: Synchronization and Observation

基本的な<em&gt;線虫Caenorhabditis elegans</em&gt;方法：同期との観測

Research into the molecular and developmental biology of the nematode Caenorhabditis elegans was begun in the early seventies by Sydney Brenner and it has ...

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

におけるプロスタグランジンの抽出と分析<em&gt;線虫（Caenorhabditis elegans）</em

Caenorhabditis elegans is emerging as a powerful animal model to study the biology of lipids1-9. Prostaglandins are an important class of eicosanoids, ...

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

多価不飽和脂肪酸の食事補給<em&gt;線虫（Caenorhabditis elegans）</em

Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, ...

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

の栽培<em&gt;線虫（Caenorhabditis elegans）</em&gt;ラボにおける三次元で

The use of genetic model organisms such as Caenorhabditis elegans has led to seminal discoveries in biology over the last five decades. Most of what we ...

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

そのまま線虫の酸素消費量の測定

Optimal mitochondrial function is critical for healthy cellular activity, particularly in cells that have high energy demands like those in the nervous ...

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

カエノハブディティス・エレガンスにおける組織特異的プロテオスタティック低下の定量化

The ability to maintain proper function and folding of the proteome (protein homeostasis) declines during normal aging, facilitating the onset of a ...

Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

Caenorhabditis elegansにおける凝集定量の自動化

A rise in the prevalence of neurodegenerative protein conformational diseases (PCDs) has fostered a great interest in this subject over the years. This ...